Tian Rui, Chen Junyu, Li Duo, Sun Xuehua, Ma Hongyan
School of Chemistry and Chemical Engineering, Yan'an University, Yan'an, Shannxi 716000, PR China.
School of Chemistry and Chemical Engineering, Yan'an University, Yan'an, Shannxi 716000, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jan 15;305:123417. doi: 10.1016/j.saa.2023.123417. Epub 2023 Sep 15.
Although the synthesis and applications of the metal nanoclusters and silica coated metal noclusters were widely explored, however, the fluorescence sensing application of silica coated metal nanoclusters are still challenges. In this work, the composite nanoparticles (PEI-Ag NCs/CSNPs) were synthesized by coating polyethyleneimine capped silver nanoclusters (PEI-Ag NCs) with chitosan/silica through a reverse microemulsion method, and then used for fluorescence enhancement detection of berberine hydrochloride (BRH). UV-vis absorption spectra, fluorescence spectra, IR spectra, transmission electron microscope (TEM) and X-ray Photoelectron Spectroscopy (XPS) technique were used to reveal the possible binding relationship between PEI-AgNCs and chitosan/silica and fluorescence sensing mechanism of PEI-Ag NCs/CSNPs to BRH. The results showed that PEI-Ag NCs/CSNPs possess better sensing ability as compared to the free PEI- Ag NCs and can be successfully applied to evaluate the BRH content in actual medicine.
尽管金属纳米团簇及二氧化硅包覆的金属纳米团簇的合成与应用已得到广泛探索，然而，二氧化硅包覆的金属纳米团簇的荧光传感应用仍面临挑战。在本工作中，通过反相微乳液法用壳聚糖/二氧化硅包覆聚乙烯亚胺封端的银纳米团簇（PEI-Ag NCs）合成了复合纳米粒子（PEI-Ag NCs/CSNPs），并将其用于盐酸小檗碱（BRH）的荧光增强检测。利用紫外-可见吸收光谱、荧光光谱、红外光谱、透射电子显微镜（TEM）和X射线光电子能谱（XPS）技术揭示了PEI-AgNCs与壳聚糖/二氧化硅之间可能的结合关系以及PEI-Ag NCs/CSNPs对BRH的荧光传感机制。结果表明，与游离的PEI-Ag NCs相比，PEI-Ag NCs/CSNPs具有更好的传感能力，并且可以成功应用于评估实际药物中的BRH含量。
