Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Palo Alto, California, U.S.A.
Department of Neurosurgery, Stanford University School of Medicine, Palo Alto, California, U.S.A.
Laryngoscope. 2024 Mar;134 Suppl 5:S1-S14. doi: 10.1002/lary.31067. Epub 2023 Sep 30.
This study compared the immune-related secretory capacity of human vestibular schwannoma (VS) and tumor-assisted macrophages (TAMs) with their normal counterparts (Schwann cells [SC] and peripheral blood monocyte-derived macrophages [Mo-MFs], respectively), and examined relationships with presurgical hearing and tumor size.
VS tumors (n = 16), auditory nerve (n = 1), blood (n = 9), and great auricular nerves (n = 3) were used. SCs (S100B ) and TAMs (CD68 ) were isolated from VS tissue for culture. The secreted levels of 65 immune-related factors were measured and compared using unpaired t-tests with Welch correction (schwannoma vs. SCs) or Mann-Whitney tests (TAMs and Mo-MFs). Associations between factor concentration and word recognition (WR), pure-tone average (PTA), and tumor size were evaluated with Spearman correlation.
Secreted factors with significantly higher concentrations in schwannoma versus SC supernatants included IL-2 and BAFF, whereas MMP-1, IL-6, FGF-2, VEGF-A, MIP-3α, and GRO-α concentrations were significantly higher in TAMs versus Mo-MFs (all p < 0.05). Worse WR was significantly associated with higher secretion of fractalkine, eotaxin-3, CD30, and IL-16 by VS cells; IP-10, eotaxin-3, multiple interleukins, GM-CSF, SCF, and CD30 by TAMs; and TNF-α and MIP-1α by Mo-MFs (all p < 0.05). Worse PTA was significantly correlated with higher secretion of IL-16 by VS cells (p < 0.05). Larger tumor size was significantly correlated with higher secretion of eotaxin by VS cells, and of IL-7, IL-21, and LIF by TAMs (all p = 0.017).
Differential secretion of immune-related factors was observed in schwannoma versus normal SCs and in TAMs versus Mo-MFs, some of which were correlated with worse hearing and larger VS tumors.
N/A Laryngoscope, 134:S1-S14, 2024.
本研究比较了人前庭神经鞘瘤（VS）与肿瘤辅助巨噬细胞（TAMs）的免疫相关分泌能力与其正常对应物（雪旺细胞[SCs]和外周血单核细胞衍生的巨噬细胞[Mo-MFs]），并研究了其与术前听力和肿瘤大小的关系。
使用 16 例 VS 肿瘤、1 例听神经、9 例血液和 3 例耳大神经。从 VS 组织中分离 SCs（S100B）和 TAMs（CD68）进行培养。使用未配对 t 检验（schwannoma vs. SCs）或 Mann-Whitney 检验（TAMs 和 Mo-MFs）比较 65 种免疫相关因子的分泌水平。使用 Spearman 相关性评估因子浓度与言语识别（WR）、纯音平均听阈（PTA）和肿瘤大小之间的关系。
与 SCs 上清液相比，雪旺细胞瘤中分泌浓度明显更高的因子包括 IL-2 和 BAFF，而 MMP-1、IL-6、FGF-2、VEGF-A、MIP-3α 和 GRO-α在 TAMs 中的浓度明显高于 Mo-MFs（均 p < 0.05）。WR 越差与 VS 细胞分泌的 fractalkine、eotaxin-3、CD30 和 IL-16 显著相关；TAMs 分泌的 IP-10、eotaxin-3、多种白细胞介素、GM-CSF、SCF 和 CD30；Mo-MFs 分泌的 TNF-α和 MIP-1α（均 p < 0.05）。PTA 越差与 VS 细胞分泌的 IL-16 显著相关（p < 0.05）。肿瘤越大与 VS 细胞分泌的 eotaxin 以及 TAMs 分泌的 IL-7、IL-21 和 LIF 显著相关（均 p = 0.017）。
在雪旺细胞瘤与正常 SCs 之间以及 TAMs 与 Mo-MFs 之间观察到免疫相关因子的差异分泌，其中一些与听力下降和 VS 肿瘤增大有关。
无。喉镜，134：S1-S14，2024。
