Fisher Melanie, Duhon Bailey H, Nguyen Han T N, Tonniges Jeffrey R, Wu Kyle C, Ren Yin
Department of Otolaryngology-Head and Neck Surgery, Division of Otology, Neurotology and Cranial Base Surgery, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.
Campus Microscopy and Imaging Facility, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio, USA.
Otolaryngol Head Neck Surg. 2025 Feb;172(2):614-622. doi: 10.1002/ohn.1018. Epub 2024 Nov 7.
The primary objective was to characterize the abundance and architecture of collagen in the extracellular matrix in vestibular schwannoma (VS). The secondary objective was to investigate the association between collagen architecture and tumor size.
Retrospective cohort study.
Academic referral center.
Tumor samples were obtained from patients with sporadic VS undergoing microsurgical resection. Histological analyses were performed including picrosirius red (PSR) staining under polarized light. Collagen architecture was quantified using an automated fiber detection software. Second Harmonic Generation (SHG) microscopy and immunofluorescence (IF) were utilized to characterize collagen architecture.
Eleven tumor specimens were included (mean tumor diameter = 2.80 cm, range 1.5-4.0 cm), and were divided into large (mean diameter = 3.5 ± 0.4 cm) and small (mean tumor diameter = 2.0 ± 0.4 cm) cohorts based on size. The large VS cohort showed significantly higher collagen density (27.65% vs 12.73%, P = .0043), with more thick fibers (mature Type I, 24.54% vs 12.97%, P = .0022) and thin fibers (immature Type I or mature Type III, 23.55% vs 12.27%, P = .026). Tumor volume correlated with greater degree of collagen fiber disorganization (P = .0413, r = 0.298). Specifically, collagen type I intensity was significantly higher in large VS compared to small tumors (P < .001) and peripheral nerve (P = .028).
Larger VS exhibit increased collagen abundance in the tumor stroma, and a more disorganized collagen architecture compared to smaller VS and normal peripheral nerve tissue. This finding indicates that collagen organization may play a significant role in extracellular matrix remodeling and the progression of VS.
主要目的是描述前庭神经鞘瘤（VS）细胞外基质中胶原蛋白的丰度和结构。次要目的是研究胶原蛋白结构与肿瘤大小之间的关联。
回顾性队列研究。
学术转诊中心。
从接受显微手术切除的散发性VS患者中获取肿瘤样本。进行了组织学分析，包括偏振光下的苦味酸天狼星红（PSR）染色。使用自动纤维检测软件对胶原蛋白结构进行量化。利用二次谐波产生（SHG）显微镜和免疫荧光（IF）来表征胶原蛋白结构。
纳入了11个肿瘤标本（平均肿瘤直径 = 2.80 cm，范围1.5 - 4.0 cm），并根据大小分为大（平均直径 = 3.5 ± 0.4 cm）和小（平均肿瘤直径 = 2.0 ± 0.4 cm）两组。大VS组显示胶原蛋白密度显著更高（27.65% 对12.73%，P = 0.0043），有更多粗纤维（成熟I型，24.54% 对12.97%，P = 0.0022）和细纤维（未成熟I型或成熟III型，23.55% 对12.27%，P = 0.026）。肿瘤体积与胶原蛋白纤维无序程度增加相关（P = 0.0413，r = 0.298）。具体而言，与小肿瘤（P < 0.001）和周围神经（P = 0.028）相比，大VS中I型胶原蛋白强度显著更高。
与较小的VS和正常周围神经组织相比，较大的VS在肿瘤基质中表现出胶原蛋白丰度增加，且胶原蛋白结构更无序。这一发现表明胶原蛋白组织可能在细胞外基质重塑和VS进展中起重要作用。
