Department of Biotechnology, Delhi Technological University (DTU), Shahbad Daulatpur Village, Bawana Road, Delhi, 110042, India.
Appl Biochem Biotechnol. 2024 May;196(5):2451-2465. doi: 10.1007/s12010-023-04741-1. Epub 2023 Sep 30.
Polymers of hydroxy alkanoates (PHA), also known as biodegradable, biocompatible plastic, are potential alternatives to petrochemical-based plastics. PHA is synthesized by microbes in their cytoplasm in the form of inclusion bodies in stress conditions such as nitrogen, oxygen, and phosphorus with excessive amounts of carbon. Sugar extracted from potato peel in the form of hydrolysate was employed as a carbon source for PHA production after acidic hydrolysis. The acid hydrolysis conditions are optimized for dilute acid concentrations and temperatures. The highest sugar-yielding condition (2% 15 min at 121 ℃) was used for submerged fermentation for PHA production by Bacillus circulans MTCC 8167. Fourier transform infrared spectroscopy, nuclear magnetic resonance, and differential scanning calorimetry were used for polymer characterization. Gas chromatography coupled with mass spectrometry confirmed the monomers such as hexadecenoic acid 3-hydroxy, methyl esters, pentadecanoic acid 14 methyl esters, and tetradecanoic acid 12- methyl esters. Crotonic acid assay was used for quantification of PHA and it was found highest (0.232 ± 0.04 g/L) at 37 °C and 36 h of incubation. Hence, potato peel waste could be a potential feedstock for waste to valuable production.
羟基烷酸聚合物（PHA），也称为可生物降解、生物相容塑料，是石油化工塑料的潜在替代品。在氮、氧、磷等应激条件下，微生物会在细胞质中以包含体的形式合成 PHA，此时碳的含量过高。经过酸性水解后，以水解物形式从土豆皮中提取的糖被用作 PHA 生产的碳源。优化了酸水解条件以适应稀酸浓度和温度。采用最高产糖条件（121℃下 2% 15 分钟）对 Bacillus circulans MTCC 8167 进行发酵，生产 PHA。采用傅里叶变换红外光谱、核磁共振和差示扫描量热法对聚合物进行了表征。气相色谱-质谱联用仪证实了单体如十六烯酸 3-羟基甲酯、十五烷酸 14 甲酯和十四烷酸 12-甲酯。使用巴豆酸测定法对 PHA 进行定量，在 37°C 和 36 小时的孵育时间下，PHA 的产量最高（0.232±0.04 g/L）。因此，土豆皮废料可以成为从废物中生产有价值产品的潜在原料。
