犬角膜内皮对用盐溶液、平衡盐溶液和含谷胱甘肽的平衡盐溶液进行眼内冲洗的反应。

Response of the canine corneal endothelium to intraocular irrigation with saline solution, balanced salt solution, and balanced salt solution with glutathione.

作者信息

Nasisse M P, Cook C S, Harling D E

出版信息

Am J Vet Res. 1986 Oct;47(10):2261-5.

Abstract

The right or left anterior chamber of 12 dogs (ie, 24 eyes) was irrigated with 100 ml of 0.9% saline solution, balanced salt solution, or balanced salt solution with glutathione (8 eyes/solution) for 22 minutes. Changes in endothelial cell density and corneal thickness from pretreatment values were evaluated immediately after, and at 2 and 7 days after irrigation. Using specular microscopy and scanning and transmission electron microscopy, corneas were examined immediately after and at 2, 7, and 14 days after irrigation. Changes were not seen in endothelial cell density after irrigation with any of the solutions evaluated. Corneal thickness increased 4% immediately after irrigation with 0.9% saline solution and returned to normal by 1 week after irrigation; treatment with 0.9% saline solution was not associated with visible corneal edema. Ultrastructural changes attributable to differences in irrigating solution composition were not observed. The difference between the endothelium's response to limited irrigation with saline solution, balanced salt solution, and balanced salt solution with glutathione was small and irrigation with these solutions was not associated with permanent endothelial damage.

摘要

用100毫升0.9%生理盐水、平衡盐溶液或含谷胱甘肽的平衡盐溶液（每种溶液处理8只眼）对12只犬（即24只眼）的右前房或左前房进行冲洗，持续22分钟。在冲洗后即刻、冲洗后2天和7天评估内皮细胞密度和角膜厚度相对于预处理值的变化。在冲洗后即刻、冲洗后2天、7天和14天，使用镜面显微镜以及扫描和透射电子显微镜对角膜进行检查。在所评估的任何一种溶液冲洗后，内皮细胞密度均未见变化。用0.9%生理盐水冲洗后，角膜厚度即刻增加4%，冲洗后1周恢复正常；用0.9%生理盐水处理未伴有可见的角膜水肿。未观察到因冲洗液成分不同而导致的超微结构变化。内皮细胞对生理盐水、平衡盐溶液和含谷胱甘肽的平衡盐溶液有限冲洗的反应差异很小，用这些溶液冲洗未伴有永久性内皮损伤。