College of Materials Science and Engineering, Hunan University, Changsha 410072, PR China.
Department of Vascular Surgery, Xiangya Hospital, Central South University, Changsha 410008, PR China.
Colloids Surf B Biointerfaces. 2023 Nov;231:113537. doi: 10.1016/j.colsurfb.2023.113537. Epub 2023 Sep 9.
Periosteum, the thin layer covering adjacent to bone containing specific architecture, is important for functional bone regeneration and remodeling. Synthetic periosteum investigated presently lacks the resemblance of natural periosteum, suffering from poor mechanical strength and cell attachment. Here, we report a newly-developed biomimetic film to function as synthetic periosteum. Based on poly(ε-caprolactone) (PCL), where surface wettability of the synthetic periosteum is enhanced by microtantalum (mTa) particle blending and after a cold drawing process, further obtains topographical anisotropy without any involvement of solvent. This new blend shows mechanical enhancement over pure PCL, with yield stress and elastic strain approaching the natural periosteum. A distinct degradation mechanism is proposed for the blend, and by seeding with mouse calvarial preosteoblasts, cell proliferation is promoted on surface of the drawn PCL but delayed on the mTa-blended PCL. However, cell mineralization is accelerated on the mTa-blended surface. This is less on the drawn PCL. The synergistical integration of cellular proliferation, alignment and osteogenic enhancement suggest that the cold drawn PCL/Ta blend has unique potential for developing into a synthetic periosteum and other tissue-engineering products.
骨膜是覆盖在骨旁的一层薄组织，具有特定的结构，对于功能性骨再生和重塑非常重要。目前研究的合成骨膜缺乏天然骨膜的相似性，存在机械强度差和细胞黏附性差等问题。在这里，我们报告了一种新开发的仿生膜，可作为合成骨膜。该仿生膜以聚己内酯（PCL）为基础，通过混合微钽（mTa）颗粒和冷拉伸工艺来提高合成骨膜的表面润湿性，从而获得无需使用溶剂的各向异性形貌。与纯 PCL 相比，这种新的共混物具有更高的机械增强性能，屈服应力和弹性应变接近天然骨膜。提出了一种新的共混物降解机制，并通过接种小鼠颅骨前成骨细胞，发现拉伸 PCL 表面上的细胞增殖得到了促进，而 mTa 共混 PCL 上的细胞增殖则被延迟。然而，mTa 共混表面上的细胞矿化加速了。在拉伸 PCL 上则较少。细胞增殖、排列和成骨增强的协同整合表明，冷拉伸 PCL/Ta 共混物具有开发合成骨膜和其他组织工程产品的独特潜力。
