Professor Dr Khadija A Jhuma, Professor & Head, Department of Biochemistry, Dhaka Medical College, Dhaka, Bangladesh; E-mail:
Mymensingh Med J. 2023 Oct;32(4):1149-1155.
As limited or no studies on pro-inflammatory cytokines (IL-1, IL-6, TNF-α)) and anti-inflammatory cytokines (IL-4, IL-10, IL-13) were reported in type 2 diabetes mellitus (T2DM) from Bangladesh. This case-control prospective interventional study was therefore undertaken to ascertain the status of them newly diagnosed Bangladeshi patients with T2DM at Medical Research Unit (MRU), MCW&H Building, MHWT, Uttara, Dhaka, Bangladesh from July 2018 to June 2021. Suspected patients with T2DM had undergone oral glucose tolerance test (OGTT) after overnight fasting. Blood samples were collected from patients at 'O' minute (Fasting) and at 2Hr (120 min) of OGTT. Newly OGTT positive 36 adult Bangladeshi patients (Male: 15, Female: 21, Age range: 35-65 years; mean±SD: 47±18 years) and 30 normal healthy subjects (Male: 12, Female: 18; Age range: 32-67 years; mean ±SD: 45±16 years) were included in the study. Serum levels of pro-inflammatory and anti-inflammatory cytokines were determined by enzyme immunoassay methods using research kits obtained from reputed companies at Medical Research Unit laboratory, Uttara, Dhaka, Bangladesh; Fasting serum levels of pro-inflammatory cytokines (IL-1, IL-6, TNF-α) were much elevated in patients compared to controls by Students t-test (p<0.001); At 2Hr of OGTT, the serum levels of these cytokines in patients remained elevated even at 2Hr of OGTT compared to their fasting levels (p<0.001). Among the anti-inflammatory cytokines, serum IL-4 level was found to be higher in patients compared to controls (p<0.05). Interestingly, F-IL-10 and F-IL-13 levels were significantly lower in patients compared to controls (p<0.001). These anti-inflammatory cytokines (IL-10 and IL-13) at 2Hr of OGTT also remained low among patients as revealed by Paired t-test (p?0.05); These results reported for the first time in Bangladeshi patients supported the concept that T2DM possibly develops due to imbalance among pro-inflammatory and anti-inflammatory cytokines. Further studies are warranted along this line involving more respondents.
由于在孟加拉国的 2 型糖尿病（T2DM）患者中，有关促炎细胞因子（IL-1、IL-6、TNF-α）和抗炎细胞因子（IL-4、IL-10、IL-13）的研究很少或没有报道。因此，本病例对照前瞻性干预研究在 2018 年 7 月至 2021 年 6 月期间，在孟加拉国达卡 Uttara 的 MCW&H 大楼的医学研究单位（MRU），对新诊断为 T2DM 的孟加拉国患者进行了这些细胞因子的状态评估。疑似 T2DM 患者在禁食过夜后接受口服葡萄糖耐量试验（OGTT）。在患者的“O”分钟（空腹）和 OGTT 的 2 小时（120 分钟）时采集血样。新的 OGTT 阳性的 36 名成年孟加拉国患者（男性 15 名，女性 21 名，年龄范围：35-65 岁；平均±SD：47±18 岁）和 30 名正常健康受试者（男性 12 名，女性 18 名；年龄范围：32-67 岁；平均±SD：45±16 岁）被纳入研究。在达卡 Uttara 的医学研究单位实验室，使用来自知名公司的研究试剂盒，通过酶免疫测定方法测定促炎和抗炎细胞因子的血清水平；与对照组相比，患者的空腹促炎细胞因子（IL-1、IL-6、TNF-α）水平通过学生 t 检验显著升高（p<0.001）；在 OGTT 的 2 小时时，与空腹水平相比，这些细胞因子的血清水平在患者中即使在 OGTT 的 2 小时仍保持升高（p<0.001）。在抗炎细胞因子中，与对照组相比，患者的血清 IL-4 水平较高（p<0.05）。有趣的是，与对照组相比，患者的 F-IL-10 和 F-IL-13 水平明显降低（p<0.001）。通过配对 t 检验（p？0.05），在患者中，OGTT 时的这些抗炎细胞因子（IL-10 和 IL-13）也保持较低水平；这些首次在孟加拉国患者中报告的结果支持 T2DM 可能是由于促炎和抗炎细胞因子之间失衡而发展的概念。沿着这条线需要进一步的研究，涉及更多的参与者。
