Barnouin J, el Idilbi N, Chilliard Y, Chacornac J P, Lefaivre R
Ann Rech Vet. 1986;17(2):129-39.
An automated method for the determination of plasma 3-hydroxybutyrate (3-HB) is described. The enzymatic principle of Williamson and Mellanby is applied to a discrete computerized analyzer (Isamat, Isa-Biologie, 94230 Cachan, France). Using this method, a single operator can determine each day the 3-HB content of 500-600 bovine plasma samples, without perchloric acid precipitation and without adding hydrazine. Aceto-acetate and acetone don't interfere in 3-HB determination. Addition of oxalate (5 mM) in Tris buffer abolishes the interference of lactate dehydrogenase (LDH). The method is accurate, with a precision of 2.8%, linear over the range 0.05-8 mM, low in cost because it needs small volumes of reagent, and correlates well with results from standard end-point manual method (common variance between the two methods: 94-98%). The method allows to use plasma 3-HB determination for assessing nutritional state of cow herds after a convenient standardization of sampling.
本文描述了一种测定血浆3-羟基丁酸（3-HB）的自动化方法。威廉姆森和梅兰比的酶法原理应用于离散式计算机分析仪（Isamat，Isa-Biologie，法国卡尚94230）。使用该方法，单个操作人员每天可测定500-600份牛血浆样本中的3-HB含量，无需高氯酸沉淀，也无需添加肼。乙酰乙酸和丙酮不干扰3-HB的测定。在Tris缓冲液中加入草酸盐（5 mM）可消除乳酸脱氢酶（LDH）的干扰。该方法准确，精密度为2.8%，在0.05-8 mM范围内呈线性，由于所需试剂量少，成本低，且与标准终点手工法的结果相关性良好（两种方法的共同方差：94-98%）。该方法允许在对采样进行适当标准化后，使用血浆3-HB测定来评估牛群的营养状况。
