Department of Biochemistry, Panjab University, Chandigarh 160014, India.
Department of Biophysics, Panjab University, Chandigarh 160014, India.
J Trace Elem Med Biol. 2023 Dec;80:127305. doi: 10.1016/j.jtemb.2023.127305. Epub 2023 Sep 16.
A balanced diet containing selenium (Se) and other trace elements is essential for normal development and growth. Se has been recognized as an essential trace element; however, its interaction with other elements has not been fully investigated. In the present study, sodium (Na), magnesium (Mg), potassium (K), calcium (Ca), chromium (Cr), manganese (Mn), iron (Fe), cobalt (Co), copper (Cu), zinc (Zn), Se and rubidium (Rb), were analysed in liver and brain regions under altered dietary Se intake in weanling mice to identify major discriminatory elements.
The study investigated the effects of different levels of Se intake on the elemental composition in liver and brain tissues of weaned mice. After 24 weeks of feeding with Se adequate, deficient, and excess diets, elemental analysis was performed on the harvested tissues using Inductively coupled plasma mass spectrometry (ICP-MS). Statistical analysis that included analysis of covariance (ANCOVA), correlation coefficient analysis, principal component analysis, and partial least squares discriminant analysis were performed.
The ANCOVA showed statistically significant changes and correlations among the analysed elements under altered dietary Se status. The multivariate analysis showed differential changes in elements in liver and brain regions. The results suggest that long-term dietary Se alternations lead to dyshomeostasis in trace elements that are required in higher concentrations compared to Se. It was observed that changes in the Fe, Co, and Rb levels were similar in all the tissues studied, whereas the changes in Mg, Cr, and Mn levels were different among the tissues under altered dietary Se status. Additionally, the changes in Rb levels correlated with the dietary Se intake but had no relation with the tissue Se levels.
The findings suggest interactions between Mg, Cr, Mn, Fe, Co, and Se under altered Se status may impact cellular functions during postnatal development. However, the possible biological significance of alterations in Rb levels under different dietary Se paradigms needs to be further explored.
含有硒（Se）和其他微量元素的均衡饮食对正常发育和生长至关重要。硒已被认为是一种必需的微量元素，但它与其他元素的相互作用尚未得到充分研究。在本研究中，我们分析了断奶小鼠在改变膳食 Se 摄入时肝脏和脑组织中钠（Na）、镁（Mg）、钾（K）、钙（Ca）、铬（Cr）、锰（Mn）、铁（Fe）、钴（Co）、铜（Cu）、锌（Zn）、硒和铷（Rb）的含量，以确定主要的区分元素。
本研究探讨了不同水平的 Se 摄入对断奶小鼠肝脏和脑组织元素组成的影响。在摄入适量、缺乏和过量 Se 饮食 24 周后，使用电感耦合等离子体质谱（ICP-MS）对收获的组织进行元素分析。进行了包括协方差分析（ANCOVA）、相关系数分析、主成分分析和偏最小二乘判别分析在内的统计分析。
ANCOVA 显示，在改变的膳食 Se 状态下，分析元素之间存在统计学上显著的变化和相关性。多元分析显示，肝脏和脑组织中元素的差异变化。结果表明，长期的膳食 Se 变化导致必需微量元素的动态平衡失调，这些元素的浓度要求高于 Se。观察到，Fe、Co 和 Rb 水平的变化在所有研究的组织中都相似，而在改变的膳食 Se 状态下，Mg、Cr 和 Mn 水平的变化在组织之间不同。此外，Rb 水平的变化与膳食 Se 摄入有关，但与组织 Se 水平无关。
研究结果表明，在改变的 Se 状态下，Mg、Cr、Mn、Fe、Co 和 Se 之间可能存在相互作用，这可能会影响出生后发育过程中的细胞功能。然而，不同膳食 Se 范式下 Rb 水平变化的可能生物学意义需要进一步探索。
