Department of Biophysics, University of Mumbai, Vidyanagari, Santacruz, Mumbai 400098, India.
Department of Physics, Guru Nanak College, Sion, Mumbai 400037, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jan 15;305:123469. doi: 10.1016/j.saa.2023.123469. Epub 2023 Sep 27.
Targeted drug delivery (TDD) is a method of delivering optimum concentrations of pharmaceutical substances in the tissue to achieve the desired therapeutic effect. Hence, TDD systems are considered as an emerging strategy to deliver the drug at the specific site of the tissues/cells. The nanoparticle-protein corona as a drug delivery vehicle has demonstrated immense benefits including potential theragnostic, improved pharmacodynamics and targeted drug delivery. In the present investigation, efforts have been to establish stable and functionalized Bovine serum albumin-gold nanoparticle (BSA-GNP) corona (conjugates) using a Direct Current (DC) electric field. With the application of DC electric field (DEF) across the BSA-GNP solution, the formation of BSA-GNP corona/conjugate takes place which was characterized using various biophysical techniques such a Dynamic Light Scattering (DLS), UV Visible spectroscopy, Fluorescence spectroscopy, electrophoresis, etc. Furthermore, the DEF engineered BSA-GNP corona was loaded/interacted with curcumin (CUR). The size of the BSA-GNP corona was increased with increasing DC voltage (5-30 V) at constant concentration of BSA. The strong and stable binding of curcumin with BSA-GNP corona was revealed by the techniques used in the investigation; however, binding affinity of CUR was decreased for 30 V DEF exposed BSA-GNP conjugate. The biocompatible experimental data confirms the nontoxic nature of BSA-GNP corona. This investigation adds a new and novel physical method for the preparation of protein-nanoparticle corona for various applications including drug delivery.
靶向药物输送（TDD）是一种在组织中输送最佳药物浓度以达到预期治疗效果的方法。因此，TDD 系统被认为是一种将药物递送到组织/细胞特定部位的新兴策略。纳米颗粒-蛋白质电晕作为药物递送载体具有巨大的益处，包括潜在的治疗学、改善的药效学和靶向药物递送。在本研究中，努力使用直流（DC）电场建立稳定和功能化的牛血清白蛋白-金纳米颗粒（BSA-GNP）电晕（缀合物）。通过在 BSA-GNP 溶液上施加直流电场（DEF），形成 BSA-GNP 电晕/缀合物，使用各种生物物理技术（如动态光散射（DLS）、紫外可见光谱、荧光光谱、电泳等）对其进行表征。此外，用 DEF 工程化的 BSA-GNP 电晕负载/与姜黄素（CUR）相互作用。随着 BSA 浓度不变，BSA-GNP 电晕的尺寸随着 DC 电压（5-30V）的增加而增加。研究中使用的技术揭示了 CUR 与 BSA-GNP 电晕的强而稳定的结合；然而，暴露于 30V DEF 的 BSA-GNP 缀合物的 CUR 结合亲和力降低。生物相容性实验数据证实了 BSA-GNP 电晕的非毒性。这项研究为各种应用（包括药物递送）的蛋白质-纳米颗粒电晕的制备增加了一种新的物理方法。
