Jaiswal Vinod D, Dongre P M
Department of Biophysics, University of Mumbai, Vidyanagari, Santacruz, Mumbai, 400098, India.
J Pharm Anal. 2020 Apr;10(2):164-177. doi: 10.1016/j.jpha.2020.02.004. Epub 2020 Feb 20.
Active targeted drug delivery methods facilitate effective uptake of functionalized nanoparticles through receptor-mediated transcytosis. In recent years, albumin-nanoparticle interaction has been critically examined so that this functionalized nanoparticle can be efficiently loaded with drugs. The present investigation aims at understanding the adsorption of Bovine Serum Albumin (BSA) on Silver Nanoparticle (SNP) surface, preparation of soft conjugates (SC) and hard conjugates (HC) of BSA-functionalized SNP (SNP-BSA), and their interaction with curcumin (CUR). HC contains tightly bound BSA whereas SC involves tightly and loosely bound BSA. Increase in the hydrodynamic radii of conjugates was observed upon SNP incubation with increased concentration of BSA. Three different SNP-BSA conjugate ratios were selected to study their interaction with CUR. Fluorescence spectroscopy showed a strong association between CUR and SNP:BSA conjugates. However, binding varied with a change in the conjugate ratio. Circular Dichroism (CD)/Fourier Transform Infrared (FTIR) spectroscopy revealed the alterations in the secondary structure of BSA upon CUR binding to the conjugates. Zeta potential data indicated stable conjugate formation. CUR in SNP:BSA conjugate was found to have a higher half-life as compared to the control. We believe that this is the first biophysical characterization report of conjugates that can be effectively extrapolated for targeted drug delivery.
主动靶向给药方法通过受体介导的转胞吞作用促进功能化纳米颗粒的有效摄取。近年来，人们对白蛋白-纳米颗粒相互作用进行了严格研究，以便这种功能化纳米颗粒能够有效地负载药物。本研究旨在了解牛血清白蛋白（BSA）在银纳米颗粒（SNP）表面的吸附情况、制备BSA功能化SNP（SNP-BSA）的软缀合物（SC）和硬缀合物（HC），以及它们与姜黄素（CUR）的相互作用。HC包含紧密结合的BSA，而SC涉及紧密结合和松散结合的BSA。随着BSA浓度增加与SNP孵育，观察到缀合物的流体力学半径增大。选择三种不同的SNP-BSA缀合比来研究它们与CUR的相互作用。荧光光谱显示CUR与SNP:BSA缀合物之间有很强的结合。然而，结合随缀合比的变化而变化。圆二色性（CD）/傅里叶变换红外（FTIR）光谱揭示了CUR与缀合物结合后BSA二级结构的改变。zeta电位数据表明形成了稳定的缀合物。与对照相比，SNP:BSA缀合物中的CUR具有更高的半衰期。我们认为这是第一份关于缀合物的生物物理表征报告，可有效地外推用于靶向给药。
