Laboratory of Tissue and Development Biology, Medicine College, Department of Health Science, Center for Biological and Health Sciences, Universidade Federal Rural do Semiárido - UFERSA, Brazil.
Laboratory of Tissue and Development Biology, Medicine College, Department of Health Science, Center for Biological and Health Sciences, Universidade Federal Rural do Semiárido - UFERSA, Brazil.
J Chem Neuroanat. 2023 Nov;133:102345. doi: 10.1016/j.jchemneu.2023.102345. Epub 2023 Sep 30.
Thimet oligopeptides (THOP 1) is a metal-dependent peptidase involved in the metabolism of neuropeptides and the presentation of peptides via MHC-1. It has been shown to play a role in the regulation of protein-protein interactions and the metabolism of intracellular peptides. THOP 1 is associated with important biological processes such as metabolism and neurodegenerative diseases, among others. The objective of this study is to elucidate the distribution of THOP 1 in the Bufo marinus brain. The analysis of THOP 1 amino acid sequences indicates that they have been conserved throughout evolution, with significant homology observed across various phyla. When comparing amphibians with other species, more than 70% identity can be identified. Immunohistochemistry analysis of the toad's brain has demonstrated that the enzyme has a ubiquitous distribution, consistent with previous findings in mammals. THOP 1 can be found in important areas of the brain, such as bulb, thalamic nuclei, striatum, hypothalamus, and among others. Nonetheless, THOP 1 is consistently localized within the nucleus, a pattern also observed in the rat brain. Therefore, based on these results, the toad appears to be an excellent model for studying the general biology of THOP 1, given the substantial homology of this enzyme with mammals and its similarity in distribution within the brain.
硫胺素寡肽 (THOP 1) 是一种金属依赖性肽酶，参与神经肽的代谢和 MHC-1 介导的肽呈递。它被证明在调节蛋白质-蛋白质相互作用和细胞内肽的代谢中发挥作用。THOP 1 与重要的生物学过程有关，如代谢和神经退行性疾病等。本研究的目的是阐明 THOP 1 在 Bufo marinus 脑中的分布。THOP 1 氨基酸序列的分析表明，它们在进化过程中得到了保守，在不同的门中观察到了显著的同源性。当将两栖动物与其他物种进行比较时，可以识别出超过 70%的同一性。对蟾蜍脑的免疫组织化学分析表明，该酶具有普遍的分布，与哺乳动物的先前发现一致。THOP 1 可以在大脑的重要区域找到，如灯泡、丘脑核、纹状体、下丘脑等。然而，THOP 1 始终定位于核内，这在大鼠脑中也观察到了这种模式。因此，基于这些结果，鉴于这种酶与哺乳动物具有高度同源性，并且在脑内的分布相似，蟾蜍似乎是研究 THOP 1 一般生物学的理想模型。
