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通过生物信息学分析和体外实验验证 PTGIS 基因在结直肠癌中的作用。

Validating the role of PTGIS gene in colorectal cancer by bioinformatics analysis and in vitro experiments.

机构信息

Medical College of Wuhan Science and Technology University, Wuhan, 430065, People's Republic of China.

Department of Immunology, School of Medicine, Jianghan University, Wuhan, 430056, People's Republic of China.

出版信息

Sci Rep. 2023 Oct 1;13(1):16496. doi: 10.1038/s41598-023-43289-2.

DOI:10.1038/s41598-023-43289-2
PMID:37779109
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10543560/
Abstract

Prostaglandin I2 synthase (PTGIS) is a member of the cytochrome P450 family. Studies have revealed that differential expression of the PTGIS gene is closely related to the pathological and physiological processes of many diseases, including breast cancer, oral squamous cell carcinoma, and head and neck cancer. However, the mechanism of action of the PTGIS gene in colorectal cancer is not fully understood. This study explored the role of PTGIS in colorectal cancer through comprehensive bioinformatics analysis and in vitro experiments, and found that the expression of PTGIS gene in colorectal cancer tissue was significantly lower than that in normal colorectal tissue (P < 0.05), and high expression of PTGIS gene was associated with poor prognosis in patients (P < 0.05). The KEGG results showed that PTGIS-related genes were mainly enriched in metabolic pathways, arachidonic acid metabolism, steroid biosynthesis, and cancer pathways. The expression of PTGIS may be related to immune infiltration. Cell experiments showed that PTGIS was expressed at a lower level in cancer. Overexpression of PTGIS inhibited apoptosis and promoted proliferation, invasion, and migration ability of SW480 colorectal cancer cells. Analysis of the PTGIS gene in this study provides a theoretical basis for further exploring the pathogenesis of colorectal cancer and finding more accurate new targets for early screening and treatment of the cancer.

摘要

前列腺素 I2 合酶（PTGIS）是细胞色素 P450 家族的成员。研究表明，PTGIS 基因的差异表达与许多疾病的病理生理过程密切相关，包括乳腺癌、口腔鳞状细胞癌和头颈部癌症。然而，PTGIS 基因在结直肠癌中的作用机制尚不完全清楚。本研究通过综合生物信息学分析和体外实验探讨了 PTGIS 在结直肠癌中的作用，发现结直肠癌组织中 PTGIS 基因的表达明显低于正常结直肠组织（P<0.05），且 PTGIS 基因高表达与患者预后不良相关（P<0.05）。KEGG 结果表明，PTGIS 相关基因主要富集在代谢途径、花生四烯酸代谢、甾体生物合成和癌症途径中。PTGIS 的表达可能与免疫浸润有关。细胞实验表明，PTGIS 在癌症中表达水平较低。过表达 PTGIS 抑制了 SW480 结直肠癌细胞的凋亡，促进了其增殖、侵袭和迁移能力。本研究对 PTGIS 基因的分析为进一步探讨结直肠癌的发病机制，寻找更准确的早期筛查和治疗结直肠癌的新靶点提供了理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/5e520f797118/41598_2023_43289_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/5bd863483ac8/41598_2023_43289_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/4c0533ebcb05/41598_2023_43289_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/4155574be5f0/41598_2023_43289_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/0520b3e93ce8/41598_2023_43289_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/1f43ec0724a0/41598_2023_43289_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/81139097bab1/41598_2023_43289_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/9b25a41b1828/41598_2023_43289_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/5e520f797118/41598_2023_43289_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/5bd863483ac8/41598_2023_43289_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/4c0533ebcb05/41598_2023_43289_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/4155574be5f0/41598_2023_43289_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/0520b3e93ce8/41598_2023_43289_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/1f43ec0724a0/41598_2023_43289_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/81139097bab1/41598_2023_43289_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/9b25a41b1828/41598_2023_43289_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a9f/10543560/5e520f797118/41598_2023_43289_Fig8_HTML.jpg

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