西安地区幽门螺杆菌分离株的抗生素耐药性与 cagA 和 vacA 基因型的关系。
Relationship between antibiotic resistance and the cagA and vacA genotypes among Helicobacter pylori strain isolates from patients in Xi'an.
机构信息
Department of Gastroenterology, the Second Affiliated Hospital of Xi'an Jiaotong University, 157 West No. 5 Road, Xi'an, 710004, Shaanxi Province, People's Republic of China.
Zhiyuan Medical Inspection Institute Co., Ltd., Hangzhou, 310009, Zhejiang Province, People's Republic of China.
出版信息
Braz J Microbiol. 2023 Dec;54(4):2773-2780. doi: 10.1007/s42770-023-01133-9. Epub 2023 Oct 2.
The issue of drug resistance of Helicobacter pylori is becoming increasingly serious. To analyze the correlation between the cagA and vacA genotypes of H. pylori strains and their resistance to metronidazole, levofloxacin, and clarithromycin in patients in Xi'an, we studied 117 H. pylori strains isolated from patients in Xi'an. Antibiotic susceptibility testing of H. pylori was performed. The cagA and vacA genotypes were investigated using PCR. Among 117 strains of H. pylori, the rate of detection of cagA was 91.45% (107/117), among which the detection rate of East Asian-type cagA was 85.05% (91/107) and that of Western-type cagA was 14.95% (16/107). There were only two genotypes of vacA: s1m1 and s1m2. The detection rate of vacAs1m1 was 47.01% (55/117) and that of vacAs1m2 was 52.99% (62/117). The dominant strains in Xi'an were cagA + vacAs1m2 strains. The metronidazole resistance rate of vacAs1m2 H. pylori strains was significantly higher than that of vacAs1m1 H. pylori strains (91.94% vs. 69.09%, P = 0.002). The levofloxacin resistance rate of Western-type cagA strains was significantly higher than that of East Asian-type cagA strains (56.25% vs. 20.88%, P = 0.004). The metronidazole resistance rate of cagA + vacAs1m2 H. pylori strains was significantly higher than that of cagA + vacAs1m1 H. pylori strains (91.23% vs. 66.00%, P = 0.001). Our results showed that Western-type cagA strains were more likely to develop levofloxacin resistance than East Asian-type cagA strains. VacAs1m2 strains were more prone to metronidazole resistance than vacAs1m1 strains.
幽门螺杆菌的耐药问题日益严重。为分析西安地区幽门螺杆菌菌株 cagA 和 vacA 基因型与对甲硝唑、左氧氟沙星和克拉霉素耐药的相关性，我们研究了西安地区 117 株幽门螺杆菌。对幽门螺杆菌进行抗生素药敏试验，采用 PCR 法检测 cagA 和 vacA 基因型。在 117 株幽门螺杆菌中，cagA 的检出率为 91.45%（107/117），其中东亚型 cagA 的检出率为 85.05%（91/107），西方型 cagA 的检出率为 14.95%（16/107）。vacA 只有 s1m1 和 s1m2 两种基因型，vacAs1m1 的检出率为 47.01%（55/117），vacAs1m2 的检出率为 52.99%（62/117）。西安地区优势菌株为 cagA+vacAs1m2 菌株。vacAs1m2 型幽门螺杆菌对甲硝唑的耐药率明显高于 vacAs1m1 型（91.94%比 69.09%，P=0.002）。西方型 cagA 菌株对左氧氟沙星的耐药率明显高于东亚型 cagA 菌株（56.25%比 20.88%，P=0.004）。cagA+vacAs1m2 型幽门螺杆菌对甲硝唑的耐药率明显高于 cagA+vacAs1m1 型（91.23%比 66.00%，P=0.001）。结果显示，西方型 cagA 菌株比东亚型 cagA 菌株更易发生左氧氟沙星耐药，vacAs1m2 菌株比 vacAs1m1 菌株更易发生甲硝唑耐药。
Zotero 插件