摩洛哥人群中甲硝唑耐药及其与毒力因子的关系。
resistance to metronidazole and its association with virulence factors in a Moroccan population.
机构信息
Laboratory of Helicobacter Pylori and Gastric Pathologies, Pasteur Institute of Morocco, Casablanca 20360, Morocco.
Faculty of Medicine of Casablanca, Ibn Rochd University Hospital Center, Hassan II University, Casablanca, Morocco.
出版信息
Pan Afr Med J. 2022 Jun 22;42:144. doi: 10.11604/pamj.2022.42.144.33217. eCollection 2022.
INTRODUCTION
surveillance data on Helicobacter pylori (H. pylori) antibiotic susceptibilities in Morocco are limited, despite resistance being the key factor in treatment failure. Virulence factors of H. pylori are associated with carcinogenesis and may be also associated with the efficacy of treatment. The aim of our study is to determine the prevalence of H. pylori metronidazole resistance in a Moroccan population infected with H. pylori and to study the impact of their virulence factors CagA and VacA on their resistance to metronidazole.
METHODS
the susceptibility to metronidazole of 185 isolates was determined by PCR. The isolates were also genotyped for CagA and VacA genes by PCR.
RESULTS
the metronidazole resistance rate was 62.70%. No association between resistance to metronidazole and social factors was detected. Regarding the virulence factors, we remarked that the moderate virulent strains s1/m2/i1/d1 with a CagA negative were the most resistant to metronidazole with a rate of 84% compared to the less virulent strains bearing the CagA negative VacA s2m2i2d2 genotype with a rate of 58% and the high virulent strains s1/m1/i1/d1-CagA positive with a rate of 47.06%.
CONCLUSION
our study revealed a very high prevalence of resistance to metronidazole in our population. The resistance ability of H. pylori maybe affected by its virulence intensity. H. pylori eradication regimens should therefore be reevaluated in this setting.
简介
尽管耐药性是治疗失败的关键因素，但摩洛哥有关幽门螺杆菌（H. pylori）抗生素敏感性的监测数据有限。H. pylori 的毒力因子与致癌作用有关，也可能与治疗效果有关。我们研究的目的是确定摩洛哥感染 H. pylori 人群中 H. pylori 甲硝唑耐药的流行率，并研究其毒力因子 CagA 和 VacA 对甲硝唑耐药性的影响。
方法
通过 PCR 法测定 185 株分离株对甲硝唑的敏感性。通过 PCR 还对 CagA 和 VacA 基因对这些分离株进行了基因分型。
结果
甲硝唑耐药率为 62.70％。未发现甲硝唑耐药性与社会因素之间存在关联。关于毒力因子，我们注意到中等毒力菌株 s1/m2/i1/d1 型 CagA 阴性对甲硝唑的耐药率最高，为 84％，而毒力较弱的菌株 CagA 阴性 VacA s2m2i2d2 基因型耐药率为 58％，毒力较强的菌株 s1/m1/i1/d1-CagA 阳性耐药率为 47.06％。
结论
我们的研究表明，在我们的人群中甲硝唑耐药率非常高。H. pylori 的耐药能力可能受其毒力强度的影响。因此，应在此背景下重新评估 H. pylori 的根除方案。
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