Kent C, Evers A, Haun S S
Arch Biochem Biophys. 1986 Nov 1;250(2):519-25. doi: 10.1016/0003-9861(86)90757-5.
Treatment of cultured cells with phospholipase C causes increased rates of hydrolysis of cellular phosphatidylcholine and increased rates of incorporation of choline into phosphatidylcholine. The fate of the diacylglycerol produced by the phospholipase C hydrolysis was examined in two cell lines, Chinese hamster ovary and HeLa. In the former cells, turnover of the glycerol moiety of phosphatidylcholine was not enhanced by phospholipase C treatment, indicating that the phospholipase C-generated diacylglycerol was recycled into new phosphatidylcholine. In HeLa cells, turnover of the glycerol backbone of phosphatidylcholine was enhanced by phospholipase C treatment, and the increased rate of turnover of the glycerol moiety was similar to that of the phosphate moiety. Thus, the fate of diacylglycerol generated at the plasma membrane was demonstrated to differ in these two cell lines. Incorporation of precursors of diacylglycerol into phosphatidylcholine was not enhanced by phospholipase C treatment in either cell line.
用磷脂酶C处理培养的细胞会导致细胞磷脂酰胆碱的水解速率增加，以及胆碱掺入磷脂酰胆碱的速率增加。在两种细胞系（中国仓鼠卵巢细胞和HeLa细胞）中研究了磷脂酶C水解产生的二酰基甘油的去向。在前者细胞中，磷脂酶C处理并未增强磷脂酰胆碱甘油部分的周转，这表明磷脂酶C产生的二酰基甘油被再循环到新的磷脂酰胆碱中。在HeLa细胞中，磷脂酶C处理增强了磷脂酰胆碱甘油主链的周转，甘油部分周转速率的增加与磷酸部分相似。因此，已证明在这两种细胞系中，质膜产生的二酰基甘油的去向不同。在任一细胞系中，磷脂酶C处理均未增强二酰基甘油前体掺入磷脂酰胆碱的过程。
