Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Faculty of Allied Health Science, Burapha University, 169 Long Had Bangsaen Rd, Saen Suk, ChonBuri District, ChonBuri 20131, Thailand.
J Agric Food Chem. 2023 Nov 1;71(43):16194-16203. doi: 10.1021/acs.jafc.3c04019. Epub 2023 Oct 2.
Lactic acid bacteria (LAB) were screened from (red sea bass), and their antimicrobial activities were evaluated against two species isolated from the , namely, (AV) and (AJ). Three LAB isolates, MU8 (EF_8), MU2 (EFL_2), and MU9 (EFL_9), were found to inhibit both AV and AJ; however, their cell-free supernatant (CFS) did not do so. Interestingly, bacteriocin-like substances (BLS) induced by cocultures of EF_8 with AV exhibited the highest antimicrobial activity against both sp. The size of BLS was less than 1.0 kDa; the purified BLS were susceptible to proteinase K digestion, indicating that they are peptides. BLS contained 13 identified peptides derived from as determined by liquid chromatography-tandem mass spectrometry. Cocultures of Gram-positive-producing and -inducing LAB strains have been used to increase bacteriocin yields. To our knowledge, this is the first report describing inducible BLS produced by cocultures of Gram-positive-producing and Gram-negative-inducing strains.
从（红笛鲷）中筛选出乳酸菌（LAB），并评估其对从 分离出的两种 物种（AV 和 AJ）的抗菌活性。发现三种 LAB 分离株 MU8（EF_8）、MU2（EFL_2）和 MU9（EFL_9）均可抑制 AV 和 AJ；然而，它们的无细胞上清液（CFS）却没有这种作用。有趣的是，EF_8 与 AV 共培养诱导的细菌素样物质（BLS）对两种 属表现出最高的抗菌活性。BLS 的大小小于 1.0 kDa；经蛋白酶 K 消化后，纯化的 BLS 表明它们是肽。通过液相色谱-串联质谱法确定，BLS 含有 13 种从 鉴定的肽。已经使用产生革兰氏阳性菌和诱导产生革兰氏阴性菌的 LAB 菌株的共培养来提高细菌素的产量。据我们所知，这是首次描述由产生革兰氏阳性菌和诱导产生革兰氏阴性菌的菌株共培养产生诱导型 BLS 的报告。
