Pilikian S, Guerin J F
Arch Androl. 1986;16(3):209-14. doi: 10.3109/01485018608986943.
Interspecies egg penetration requires completion of capacitation and acrosome reaction of the spermatozoa. The ability of frozen-thawed human semen to accomplish this reaction was studied, and its relation to the zona-free hamster egg penetration was investigated. The percentage of reacted cells in the population of live spermatozoa estimated by the triple-staining technique was found to be greater in frozen than in fresh samples. However, the egg-penetrating ability of cryopreserved semen remained significantly low. Individual differences in the ability to undergo the acrosome reaction and its relation to penetration are discussed.
种间卵子穿透需要精子完成获能和顶体反应。研究了冻融人类精液完成这种反应的能力，并调查了其与去透明带仓鼠卵子穿透的关系。通过三重染色技术估计，活精子群体中发生反应的细胞百分比在冷冻样本中比新鲜样本中更高。然而，冷冻保存精液的卵子穿透能力仍然显著较低。讨论了顶体反应能力的个体差异及其与穿透的关系。
