Critser J K, Arneson B W, Aaker D V, Huse-Benda A R, Ball G D
Fertil Steril. 1987 Jun;47(6):980-4. doi: 10.1016/s0015-0282(16)59233-4.
Postthaw dynamics of motility maintenance and ability to penetrate zona-free hamster ova were examined with human sperm. Ten semen samples were each divided into two equal volumes; one was cryopreserved while the other half remained untreated. Frozen samples were thawed, and initial evaluations for motility and hamster egg penetration were made on both untreated and frozen-thawed samples. The time difference between the initial evaluations for the two treatment groups was approximately 30 minutes as a result of the time required to freeze and thaw aliquots. Subsequent evaluations were made 6, 12, 24, and 48 hours later. Over all times both the motility and fertilizability of cryopreserved spermatozoa were significantly reduced (P less than 0.05) when compared with those of untreated sperm. The pattern of motility loss over time was similar between untreated and frozen-thawed sperm (P greater than 0.10). Conversely, differences between untreated and frozen-thawed sperm in fertilizability patterns were dramatic (P less than 0.05). This was evidenced by penetration rates for cryopreserved sperm highest at 0 hour and decreasing over time, whereas penetration by untreated spermatozoa was lowest at 0 hour, increasing to a maximum at 24 hours. These observations may be important in the development of laboratory protocols for freezing and clinical protocols for using frozen-thawed sperm.
用人精子检测了冻融后精子活力维持和穿透去透明带仓鼠卵能力的动态变化。将10份精液样本各分成两等份；一份进行冷冻保存，另一半不作处理。冷冻样本解冻后，对未处理样本和冻融样本进行了活力和仓鼠卵穿透的初始评估。由于冷冻和解冻等分样本所需的时间，两个处理组初始评估之间的时间差约为30分钟。随后在6、12、24和48小时后进行了评估。与未处理的精子相比，冷冻保存的精子在所有时间点的活力和受精能力均显著降低（P<0.05）。未处理精子和冻融精子随时间的活力丧失模式相似（P>0.10）。相反，未处理精子和冻融精子在受精能力模式上的差异很大（P<0.05）。这表现为冷冻保存精子的穿透率在0小时最高，随时间下降，而未处理精子的穿透率在0小时最低，在24小时增加到最大值。这些观察结果可能对冷冻实验室方案和使用冻融精子的临床方案的制定具有重要意义。
