Guo Weidan, Xiao Yu, Fu Xiangjin, Long Zhao, Wu Yue, Lin Qinlu, Ren Kangzi, Jiang Liwen
College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
Nutrition and Health Products Engineering Technology Research Center of Hunan Province, Changsha 410004, China.
Food Chem X. 2023 Jul 7;19:100779. doi: 10.1016/j.fochx.2023.100779. eCollection 2023 Oct 30.
In this study, the effect of Douchi extract () on α-glucosidase and angiotensin-converting enzymes (ACE) were investigated, and several novel peptides with inhibitory activity against α-glucosidase and ACE were identified using peptidomics approach based on UPLC-MS/MS. The average inhibition rates of on α-glucosidase and ACE were 73.75-78.10% and 4.56-27.07%, respectively. In the , a total of 710 peptides were detected. Two novel peptides with potential inhibitory activity against α-glucosidase were identified using the correlation analysis, database alignment and molecular docking methods. They were DVFRAIPSEVL and DRPSINGLAGAN, with the IC values of 0.121 and 0.128 mg/mL, respectively. Also, four novel peptides with potential inhibitory activity against ACE were identified: PSSPFTDLWD, EEQDERQFPF, PVPVPVQQAFPF and PSSPFTDL, with IC values of 1.388, 0.041, 0.761 and 0.097 mg/mL, respectively. These results indicated that combining peptidomics and molecular docking is an effective alternative strategy for rapidly screening numbers of novel bioactive peptides from foods.
在本研究中，研究了豆豉提取物对α-葡萄糖苷酶和血管紧张素转换酶（ACE）的作用，并基于超高效液相色谱-串联质谱（UPLC-MS/MS）的肽组学方法鉴定了几种对α-葡萄糖苷酶和ACE具有抑制活性的新型肽。豆豉提取物对α-葡萄糖苷酶和ACE的平均抑制率分别为73.75 - 78.10%和4.56 - 27.07%。在肽组分析中，共检测到710种肽。通过相关性分析、数据库比对和分子对接方法，鉴定出两种对α-葡萄糖苷酶具有潜在抑制活性的新型肽。它们分别是DVFRAIPSEVL和DRPSINGLAGAN，IC50值分别为0.121和0.128 mg/mL。此外，还鉴定出四种对ACE具有潜在抑制活性的新型肽：PSSPFTDLWD、EEQDERQFPF、PVPVPVQQAFPF和PSSPFTDL，IC50值分别为1.388、0.041、0.761和0.097 mg/mL。这些结果表明，将肽组学和分子对接相结合是从食物中快速筛选大量新型生物活性肽的有效替代策略。
