Liu Chunmei, Ding Zhenjiang, Zhang Zihan, Zhao Laiyu, Zhang Chunhui, Huang Feng
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.
Beijing Key Laboratory of the Innovative Development of Functional Staple and Nutritional Intervention for Chronic Diseases, China National Research Institute of Food and Fermentation Industries, Beijing 100015, China.
Food Chem X. 2023 Jul 25;19:100806. doi: 10.1016/j.fochx.2023.100806. eCollection 2023 Oct 30.
This study aimed to investigate how postmortem muscle cells' mitochondria changed in morphology from three aspects: the outer membrane, cristae, and fission/fusion. Atomic force microscopy (AFM) results showed that mitochondria underwent a morphology transformation from normal to swelling and collapse. Meanwhile, the cleavage of OPA1, upregulation of OMA1, downregulation of Mic60 and transmission electron microscope micrographs revealed that mitochondrial cristae ruptured with an aging time extended. Additionally, the increased expressions of Fis1 and Drp1, and the AFM topographic images mutually confirmed mitochondrial fission. These results further proved from the perspective of mitochondrial morphology that the degree of mitochondrial damage increased with the postmortem aging time extended, which was consistent with the results of the release of cytochrome caused by the increase of mitochondrial permeability transition pore opening and the decrease of mitochondrial membrane permeability, and further induced the apoptosis of postmortem muscle cells.
本研究旨在从外膜、嵴以及分裂/融合三个方面探讨死后肌肉细胞线粒体的形态变化。原子力显微镜（AFM）结果显示，线粒体经历了从正常到肿胀和塌陷的形态转变。同时，OPA1的裂解、OMA1的上调、Mic60的下调以及透射电子显微镜图像显示，随着老化时间延长，线粒体嵴破裂。此外，Fis1和Drp1表达的增加以及AFM形貌图像相互证实了线粒体分裂。这些结果从线粒体形态学角度进一步证明，随着死后老化时间延长，线粒体损伤程度增加，这与线粒体通透性转换孔开放增加和线粒体膜通透性降低导致的细胞色素释放结果一致，并进一步诱导了死后肌肉细胞的凋亡。
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