Ding Zhenjiang, Liu Chunmei, Zhang Zihan, Zhang Chunhui, Huang Feng
Beijing Key Laboratory of the Innovative Development of Functional Staple and Nutritional Intervention for Chronic Diseases, China National Research Institute of Food and Fermentation Industries, Beijing 100015, China.
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.
Food Chem X. 2024 Apr 7;22:101366. doi: 10.1016/j.fochx.2024.101366. eCollection 2024 Jun 30.
The mitochondrial calcium uniporter (MCU) occupies a noteworthy position in the regulation of mitochondrial calcium uptake. This study investigated the effects of MCU modulator-mediated mitochondrial calcium on mitochondrial dysfunction, oxidative stress, endogenous enzyme activities, and tenderness during postmortem aging. Spermine, as an activator of MCU, resulted in an increase in mitochondrial calcium levels, not only disrupting mitochondrial morphology but also triggering mitochondrial oxidative stress and downregulation of antioxidant factors. Additionally, the spermine group underwent later activation of calpain and earlier activation of caspases, as well as the myofibril fragmentation index was initially lower and then higher compared with control group, indicating that endogenous enzymes played an indispensable role in different aging periods. Interestingly, the results of the Ru360 (an inhibitor of MCU) group were opposite to those aforementioned findings. Our data provide a novel perspective on the regulatory mechanism of mitochondrial calcium homeostasis mediated by MCU on tenderness.
线粒体钙单向转运体（MCU）在调节线粒体钙摄取方面占据着重要地位。本研究调查了MCU调节剂介导的线粒体钙对宰后成熟过程中线粒体功能障碍、氧化应激、内源性酶活性和嫩度的影响。精胺作为MCU的激活剂，导致线粒体钙水平升高，不仅破坏线粒体形态，还引发线粒体氧化应激和抗氧化因子的下调。此外，与对照组相比，精胺组钙蛋白酶的激活较晚，半胱天冬酶的激活较早，且肌原纤维破碎指数最初较低，随后较高，这表明内源性酶在不同的成熟阶段发挥着不可或缺的作用。有趣的是，Ru360（一种MCU抑制剂）组的结果与上述发现相反。我们的数据为MCU介导的线粒体钙稳态对嫩度的调节机制提供了新的视角。
