Zhang Mingfei, Lu Wen, Yang Xinxia, Li Qin, Lin Xingyu, Liu Kexin, Yin Chunmei, Xiong Bo, Liao Ling, Sun Guochao, He Siya, He Jiaxian, Wang Xun, Wang Zhihui
College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
Front Plant Sci. 2023 Sep 15;14:1264283. doi: 10.3389/fpls.2023.1264283. eCollection 2023.
Sugars and organic acids are the main factors determining the flavor of citrus fruit. The WRKY transcription factor family plays a vital role in plant growth and development. However, there are still few studies about the regulation of citrus WRKY transcription factors (CsWRKYs) on sugars and organic acids in citrus fruit. In this work, a genome-wide analysis of s was carried out in the citrus genome, and a total of 81 s were identified, which contained conserved WRKY motifs. -regulatory element analysis revealed that most of the promoters contained several kinds of hormone-responsive and abiotic-responsive -elements. Furthermore, gene expression analysis and fruit quality determination showed that multiple s were closely linked to fruit sugars and organic acids with the development of citrus fruit. Notably, transcriptome co-expression network analysis further indicated that three s, namely, , , and , co-expressed with multiple genes involved in various pathways, such as Pyruvate metabolism and Citrate cycle. These CsWRKYs may participate in the metabolism of fruit sugars and organic acids by regulating carbohydrate metabolism genes in citrus fruit. These findings provide comprehensive knowledge of the family on the regulation of fruit quality.
糖类和有机酸是决定柑橘果实风味的主要因素。WRKY转录因子家族在植物生长发育中起着至关重要的作用。然而，关于柑橘WRKY转录因子（CsWRKYs）对柑橘果实中糖类和有机酸的调控研究仍然较少。在这项工作中，对柑橘基因组进行了全基因组范围的分析，共鉴定出81个CsWRKYs，它们含有保守的WRKY基序。顺式作用元件分析表明，大多数CsWRKYs启动子包含几种激素响应和非生物响应元件。此外，基因表达分析和果实品质测定表明，随着柑橘果实的发育，多个CsWRKYs与果实糖类和有机酸密切相关。值得注意的是，转录组共表达网络分析进一步表明，三个CsWRKYs，即CsWRKY28、CsWRKY33和CsWRKY53，与参与各种途径（如丙酮酸代谢和柠檬酸循环）的多个基因共表达。这些CsWRKYs可能通过调控柑橘果实中的碳水化合物代谢基因参与果实糖类和有机酸的代谢。这些发现为CsWRKYs家族对果实品质的调控提供了全面的认识。
