Sugar is a primary determinant of citrus fruit flavour, but undergoes varied accumulation processes across different citrus varieties owing to high genetic variability. Sucrose phosphate synthase (SPS), a key enzyme in glucose metabolism, plays a crucial role in this context. Despite its significance, there is limited research on sugar component quality and the expression and regulatory prediction of genes during citrus fruit development. Therefore, we analysed the sugar quality formation process in 'Kiyomi' and 'Succosa', two citrus varieties, and performed a comprehensive genome-wide analysis of citrus . We observed that the accumulation of sugar components significantly differs between the two varieties, with the identification of four in citrus. sequences were highly conserved, featuring typical SPS protein domains. Expression analysis revealed a positive correlation between expression and sugar accumulation in citrus fruits. However, expression displays specificity to different citrus tissues and varieties. Transcriptome co-expression network analysis suggests the involvement of multiple transcription factors in shaping citrus fruit sugar quality through the regulation of . Notably, the expression levels of four (, , , ), were significantly positively correlated with and might can activate sugar accumulation in citrus fruit through . Collectively, we further emphasize the potential importance of in citrus sugar metabolism, our findings serve as a reference for understanding sugar component formation and predicting expression and regulation during citrus fruit development.