Asahina Hironao, Ogasawara Tadashi, Akieda Toshie, Miyahara Kohta, Okada Yoshiyuki, Matsumura Kohei, Taniguchi Makoto, Yoshida Akihiro, Kakinoki Yasuaki
Department of Oral Health Promotion, Graduate School of Oral Medicine, Matsumoto Dental University, Nagano, 399-0704, Japan.
Department of Special Care Dentistry, Hiroshima University Hospital, Hiroshima, 734-8551, Japan.
Heliyon. 2023 Sep 23;9(10):e20401. doi: 10.1016/j.heliyon.2023.e20401. eCollection 2023 Oct.
Tube feeders are prone to membranous substance formation on the palate, and those with membranous substances have a risk of fever, with the probable involvement of their oral bacteria. However, the palatal microbiota of those with membranous substances has not been elucidated. Therefore, we evaluated the differences in palatal microbiota between tube-fed individuals with and without membranous substances to clarify the microbiota.
This study included 19 participants aged 65 years who required tube feeding. The participants' characteristics were collected from nursing records and oral examinations. If membranous materials were found on the palate, a specimen was collected. Membranous substances were defined as keratotic degeneration observed under a microscope. Additionally, we performed a comprehensive microbiome analysis by extracting DNA from the samples and performing 16 S rRNA gene sequencing. Finally, we compared the participant demographics and oral microbiota between patients with and without membranous substances.
A total of 11 participants had membranous substances associated with "mouth dryness" (p < 0.001) and "constant mouth opening" (p = 0.020). Palatal microbiota differed between those with and without membranous substances. Among the bacteria with a relative abundance greater than 1.0%, the abundance of (p = 0.007), (p = 0.041), (p = 0.009), and subsp. (p = 0.026) was significantly higher in the membranous substance group than in the non-membranous substance group.
The palatal microbiota of individuals undergoing tube feeding differed depending on the presence or absence of membranous substances. Membrane substance formation associated with dry mouth purportedly alters the palatal microbiota. , , . , and . subsp. were more abundant in the oral microbiota of patients with membranous substances. Thus, preventing this formation may help in controlling the growth of these microbes.
鼻饲管使用者的上腭容易形成膜状物质，有膜状物质的使用者存在发热风险，可能与口腔细菌有关。然而，有膜状物质者的上腭微生物群尚未阐明。因此，我们评估了有和没有膜状物质的鼻饲个体之间上腭微生物群的差异，以阐明微生物群。
本研究纳入了19名65岁需要鼻饲的参与者。从护理记录和口腔检查中收集参与者的特征。如果在上腭发现膜状物质，则采集样本。膜状物质定义为显微镜下观察到的角化变性。此外，我们通过从样本中提取DNA并进行16S rRNA基因测序进行了全面的微生物组分析。最后，我们比较了有和没有膜状物质的患者的人口统计学特征和口腔微生物群。
共有11名参与者有与“口干”（p < 0.001）和“持续张口”（p = 0.020）相关的膜状物质。有和没有膜状物质者的上腭微生物群不同。在相对丰度大于1.0%的细菌中，膜状物质组中（p = 0.007）、（p = 0.041）、（p = 0.009）和亚种（p = 0.026）的丰度显著高于无膜状物质组。
鼻饲个体的上腭微生物群因有无膜状物质而有所不同。据称与口干相关的膜状物质形成会改变上腭微生物群。、、。和。亚种在有膜状物质患者的口腔微生物群中更为丰富。因此，预防这种形成可能有助于控制这些微生物的生长。
