Darmanin Connie, Babayekhorasani Firoozeh, Formosa Andrew, Spicer Patrick, Abbey Brian
La Trobe Institute for Molecular Science, Department of Mathematical and Physical Sciences, School of Computing Engineering and Mathematical Science, La Trobe University, Bundoora, VIC 3086, Australia.
Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia; School of Chemical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
J Colloid Interface Sci. 2024 Jan;653(Pt B):1123-1136. doi: 10.1016/j.jcis.2023.09.093. Epub 2023 Sep 21.
The use of monoolein/water mixtures in serial crystallography experiments using high-viscosity injectors (HVI) results in significant departures from equilibrium behaviour. This is expected to include changes in phase, viscosity, and associated flow behaviour. It should be possible to detect these changes, in-situ, using a combination of polarisation and rheology characterisation techniques.
A systematic study was performed using monoolein, varying the water content to create a range of mixtures. Injection induced phase changes within the HVI flow were established using real-time cross-polarization measurements. Dynamic flow characteristics and viscosity was characterized by particle tracking and rheology.
HVI injection induces deformation and phase changes within monoolein (MO)/water mixtures which can be detected through variations in the transmitted intensity during in-situ polarisation studies. The heterogeneity of the extruded sample results in a highly viscous cubic phase in the central region of the stream and a less viscous lamellar-rich phase at the edges adjacent to the walls. The extent of these variations depends on sample composition and injection conditions. Shear-thinning behaviour and increasing heterogeneity in the vicinity of the capillary walls under dynamic flow conditions. This is the first report observing injection induced dynamical behaviour in MO/water mixtures under realistic flow conditions.
在使用高粘度注射器（HVI）的串行晶体学实验中使用单油酸甘油酯/水混合物会导致显著偏离平衡行为。预计这将包括相、粘度和相关流动行为的变化。应该可以使用偏振和流变学表征技术的组合原位检测这些变化。
使用单油酸甘油酯进行了一项系统研究，改变水含量以创建一系列混合物。使用实时交叉偏振测量确定HVI流内注射引起的相变。通过粒子跟踪和流变学表征动态流动特性和粘度。
HVI注射会在单油酸甘油酯（MO）/水混合物中引起变形和相变，这可以通过原位偏振研究期间透射强度的变化来检测。挤出样品的不均匀性导致流中心区域出现高粘性立方相，而在与壁相邻的边缘处出现粘性较小的富含层状相的相。这些变化的程度取决于样品组成和注射条件。在动态流动条件下，毛细管壁附近出现剪切变稀行为和不均匀性增加。这是第一份观察到在实际流动条件下单油酸甘油酯/水混合物中注射引起的动态行为的报告。
