School of Mathematics, Physics and Optoelectronic Engineering, Hubei University of Automotive Technology, Shiyan, Hubei 442002, China.
Nanchang key laboratory of animal health and safety production, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.
Poult Sci. 2023 Dec;102(12):103102. doi: 10.1016/j.psj.2023.103102. Epub 2023 Sep 11.
Hydrogen sulfide (HS) is one of the most irritant gases present in rearing stalls that suppress broilers' healthy growth, which is seriously required an effective alleviation method. In this study, Lactobacillus was supplemented to investigate the alleviative effects on broilers reared under consecutive HS exposure. A total of 180 healthy 1-day-old male AA broilers with similar body weight (40.8 ± 1.0 g) were randomly allotted into the control treatment (CON), the hydrogen sulfide treatment (HS), and the Lactobacillus supplement under HS exposure treatment (LAC) for a 42-d-long feeding process. Growth and carcass performances, immunity-related parameters, intestinal development and cecal microbial communities, and blood metabolites were measured. Results showed that Lactobacillus supplement significantly increased the body weight gain (BWG) while reduced the mortality rate, abdominal fat and bursa of fabricius weight during the whole rearing time compared with HS treatment (P < 0.05). Serum LPS, IL-1β, IL-2, and IL-6 contents were observed significantly increased after HS treatment while remarkably decreased after Lactobacillus supplementation(P < 0.05). Intestinal morphology results showed a significant higher in the development of ileum villus height (P < 0.05). Cecal microbiota results showed the bacterial composition was significantly altered after Lactobacillus supplement (P < 0.05). Specifically, Lactobacillus supplement significantly decreased the relative abundance of Faecalibacterium, while significantly proliferated the relative abundance of Lactobacillus, Bifidobacterium, Clostridium, and Campylobacter (P<0.05). Metabolic results indicated that Lactobacillus supplement may alleviate the harmful effects caused by HS through regulating the pyrimidine metabolism, starch and sucrose metabolism, fructose and mannose degradation, and beta-alanine metabolism. In summary, Lactobacillus supplement effectively increased BWG and decreased mortality rate of broilers under HS exposure by enhancing the body's immune capacity, proliferating beneficial microbes (e.g., Lactobacillus and Bifidobacterium), and regulating the physiological pyrimidine metabolism, starch and sucrose metabolism, and beta-alanine metabolism.
硫化氢（HS）是饲养舍中存在的最刺激性气体之一，会抑制肉鸡的健康生长，因此迫切需要一种有效的缓解方法。在这项研究中，添加了乳酸菌来研究其对连续暴露于 HS 下的肉鸡的缓解作用。总共选择了 180 只体重（40.8±1.0g）相似的 1 日龄雄性 AA 肉鸡，随机分为对照组（CON）、硫化氢处理组（HS）和 HS 暴露下添加乳酸菌组（LAC），进行 42 天的饲养过程。测量生长和屠体性能、免疫相关参数、肠道发育和盲肠微生物群落以及血液代谢物。结果表明，与 HS 处理相比，LAC 处理显著增加了 BWG，同时降低了死亡率、腹部脂肪和法氏囊重量（P＜0.05）。HS 处理后血清 LPS、IL-1β、IL-2 和 IL-6 含量显著增加，而添加乳酸菌后显著降低（P＜0.05）。肠道形态学结果表明，回肠绒毛高度的发育明显更高（P＜0.05）。盲肠微生物群结果表明，添加乳酸菌后细菌组成发生了显著变化（P＜0.05）。具体而言，LAC 处理显著降低了粪杆菌的相对丰度，而显著增加了乳酸菌、双歧杆菌、梭菌和弯曲杆菌的相对丰度（P＜0.05）。代谢结果表明，LAC 补充可能通过调节嘧啶代谢、淀粉和蔗糖代谢、果糖和甘露糖降解以及β-丙氨酸代谢来缓解 HS 造成的有害影响。综上所述，LAC 补充通过增强机体免疫能力、增殖有益微生物（如乳酸菌和双歧杆菌）以及调节生理嘧啶代谢、淀粉和蔗糖代谢以及β-丙氨酸代谢，有效提高了 HS 暴露下肉鸡的 BWG 并降低了死亡率。
