通过牛磺酸介导的高光条件下二次细胞壁形成抑制提高雨生红球藻虾青素的生产、回收和生物可及性。
Enhancement of astaxanthin production, recovery, and bio-accessibility in Haematococcus pluvialis through taurine-mediated inhibition of secondary cell wall formation under high light conditions.
机构信息
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315832, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315832, China.
出版信息
Bioresour Technol. 2023 Dec;389:129802. doi: 10.1016/j.biortech.2023.129802. Epub 2023 Oct 1.
This study explored the use of taurine in enhancing the production and bio-accessibility of astaxanthin in Haematococcus pluvialis, which typically forms a secondary cell wall hindering astaxanthin extraction. The biomass of taurine-treated group significantly increased by 18%, and astaxanthin yield surged by 34% in comparison to the control group. Without cell disruption, astaxanthin recovery from thin-walled cells in the taurine-treated group, using dimethyl sulfoxide and ethanol as extraction reagents, was 97% and 75%, respectively, which were 30-fold higher than those of thick-walled cells in the control group. Additionally, the cell fragmentation rate increased by 86% in taurine-treated group relative to the control group. Comparative transcriptome analysis identified taurine-induced upregulation of genes involved in the astaxanthin biosynthesis pathway and downregulation of those associated with secondary cell wall synthesis. This study thus offers an innovative taurine-based strategy to enhance astaxanthin production and bio-accessibility while shedding light on the mechanisms driving this process.
本研究探讨了牛磺酸在提高雨生红球藻中虾青素的产量和生物可及性方面的应用。雨生红球藻会形成一层次生细胞壁，从而阻碍虾青素的提取。与对照组相比，牛磺酸处理组的生物量显著增加了 18%，虾青素产量增加了 34%。未经细胞破壁处理，使用二甲基亚砜和乙醇作为提取试剂，从牛磺酸处理组的薄壁细胞中回收虾青素的回收率分别为 97%和 75%，分别是对照组厚壁细胞的 30 倍。此外，牛磺酸处理组的细胞破碎率比对照组增加了 86%。比较转录组分析鉴定出牛磺酸诱导虾青素生物合成途径相关基因的上调和与次生细胞壁合成相关基因的下调。因此，本研究提供了一种基于牛磺酸的创新策略，可提高虾青素的产量和生物可及性，并揭示了推动这一过程的机制。
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