Acheampong Adolf, Wang Rong, Elsherbiny Shereen M, Bondzie-Quaye Precious, Huang Qing
CAS Key Laboratory of High Magnetic Field and Iron Beam Physical Biology, Institute of Intelligent Machines, Hefei Institute of Physical Sciences, Chinese Academy of Sciences, Hefei 230031, China; Science Island Branch of Graduate School, University of Science and Technology of China, Hefei 230026, China.
CAS Key Laboratory of High Magnetic Field and Iron Beam Physical Biology, Institute of Intelligent Machines, Hefei Institute of Physical Sciences, Chinese Academy of Sciences, Hefei 230031, China; Science Island Branch of Graduate School, University of Science and Technology of China, Hefei 230026, China; School of Environment and Energy Engineering, Anhui Jianzhu University, Heifei 230601, China.
Bioresour Technol. 2024 Feb;393:130001. doi: 10.1016/j.biortech.2023.130001. Epub 2023 Nov 11.
The economical way of Haematococcus pluvialis farming is to simultaneously achieve biomass, astaxanthin and lipid using less expensive chemicals. This paper explores the role of exogenous arginine in promoting growth and astaxanthin accumulation under stressful conditions. The application of arginine exerts a synergic effect on biomass, astaxanthin and lipid by improving carbon utilization, activating the arginine pathway and regulating carotenoid and lipid-related genes. Genes related to arginine catabolism, such as ADC, OCT, ASS1, NOS, and OAT, were up-regulated at both the cultivation and astaxanthin induction stages, signifying their importance in both growth and astaxanthin synthesis. Furthermore, transcriptome analysis revealed that arginine up-regulated transcription levels of genes involved carbon fixing, lipid biosynthesis, pyruvate metabolism, carotenoid, tricarboxylic acid cycle, and arginine and proline metabolism. The results provide a significant mechanism and applicability of using exogenous arginine and high light to stimulate bioproducts from Haematococcus pluvialis.
雨生红球藻养殖的经济方式是使用成本较低的化学物质同时实现生物量、虾青素和脂质的产出。本文探讨了外源精氨酸在应激条件下促进雨生红球藻生长和虾青素积累中的作用。精氨酸的应用通过改善碳利用、激活精氨酸途径以及调控类胡萝卜素和脂质相关基因，对生物量、虾青素和脂质产生协同效应。与精氨酸分解代谢相关的基因，如ADC、OCT、ASS1、NOS和OAT，在培养阶段和虾青素诱导阶段均上调，表明它们在生长和虾青素合成中均具有重要作用。此外，转录组分析显示精氨酸上调了参与碳固定、脂质生物合成、丙酮酸代谢、类胡萝卜素、三羧酸循环以及精氨酸和脯氨酸代谢的基因的转录水平。这些结果为利用外源精氨酸和高光刺激雨生红球藻生物产品提供了重要机制和应用价值。
