IDEXX Laboratories, Inc., 1 IDEXX Drive, Westbrook, ME 04092, USA.
College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, 11, Raleigh, NC 27606, USA.
Vet Parasitol Reg Stud Reports. 2023 Oct;45:100923. doi: 10.1016/j.vprsr.2023.100923. Epub 2023 Aug 24.
Point-of-care (POC) ELISA tests are routinely used in US veterinary practices to screen canine patients for antibodies to tick-transmitted pathogens. Results are also used to monitor spatial and temporal trends in canine seroprevalence, and these data can build awareness of the risk to humans of tick-transmitted diseases such as Lyme disease and anaplasmosis. This study utilized a second-generation test that has incorporated additional Anaplasma-specific peptides into a commercial POC ELISA test to allow detection of Anaplasma spp. antibodies earlier post-infection. A convenience population consisting of 19,894 canine samples from a US commercial diagnostic laboratory were tested using the second-generation POC ELISA test to describe regional Anaplasma spp. canine seroprevalence and assess correlation to anaplasmosis cases reported to Centers for Disease Control and Prevention by state. Antibodies to Anaplasma spp. were detected in 1646 samples (8.3%) with the Northeast and Midwest US census regions having the highest proportion of positive samples. At the state level, a significant correlation was found between canine Anaplasma spp. seroprevalence and human anaplasmosis incidence (r = 0.64). Although estimates of canine Anaplasma spp. seroprevalence presented here using the second-generation POC ELISA are generally increased, especially in the Northeast and Midwest, the regional distribution of canine samples testing positive for Anaplasma spp. antibodies is consistent with previous reports. The observed correlation with human anaplasmosis incidence indicates that results from the second-generation POC ELISA will continue to add value in epidemiological assessment of human anaplasmosis risk.
即时检测 (POC) ELISA 试验常用于美国兽医诊所，以筛选犬类患者是否存在 tick 传播病原体的抗体。试验结果还用于监测犬类血清阳性率的时空趋势，这些数据可以提高人们对 tick 传播疾病（如莱姆病和无形体病）对人类风险的认识。本研究利用第二代检测方法，在商业 POC ELISA 检测中纳入了更多的无形体特异性肽，以更早地检测到感染后无形体的抗体。本研究使用第二代 POC ELISA 检测方法，对美国一家商业诊断实验室的 19894 份犬样本进行了检测，这些样本来自方便样本，用于描述区域内无形体的犬血清阳性率，并评估与疾病控制和预防中心报告的各州无形体病病例的相关性。在 1646 份样本（8.3%）中检测到了无形体抗体，美国东北部和中西部的普查区阳性样本比例最高。在州一级，犬无形体血清阳性率与人类无形体病发病率之间存在显著相关性（r = 0.64）。尽管使用第二代 POC ELISA 检测方法，这里提出的犬无形体血清阳性率估计值通常会增加，特别是在东北部和中西部，但犬类样本中检测到的无形体抗体阳性的区域分布与之前的报告一致。与人类无形体病发病率的观察相关性表明，第二代 POC ELISA 的检测结果将继续为人类无形体病风险的流行病学评估增加价值。
