Zhen Y S, Reardon M A, Weber G
Biochem Biophys Res Commun. 1986 Oct 15;140(1):434-9. doi: 10.1016/0006-291x(86)91109-5.
This study provides evidence that the dipyridamole inhibitory effect on nucleoside incorporation changed with culture time. Lag and log phase hepatoma 3924A cells were highly sensitive to dipyridamole with IC50 values for thymidine incorporation of 0.20 and 0.31 microM, respectively. In contrast, stationary phase cells were comparatively insensitive to dipyridamole with an IC50 of 38.9 microM. Amphotericin B (10 microM) restored the sensitivity of stationary phase cells to dipyridamole, lowering the IC50 value for thymidine incorporation to 0.25 microM. Amphotericin B also enhanced the cytotoxicity of dipyridamole to hepatoma 3924A cells. The combination of amphotericin B and dipyridamole may be useful in cancer chemotherapy.
本研究提供的证据表明，双嘧达莫对核苷掺入的抑制作用随培养时间而变化。对数期和迟滞期的肝癌3924A细胞对双嘧达莫高度敏感，胸苷掺入的IC50值分别为0.20和0.31微摩尔。相比之下，稳定期细胞对双嘧达莫相对不敏感，IC50为38.9微摩尔。两性霉素B（10微摩尔）恢复了稳定期细胞对双嘧达莫的敏感性，使胸苷掺入的IC50值降至0.25微摩尔。两性霉素B还增强了双嘧达莫对肝癌3924A细胞的细胞毒性。两性霉素B和双嘧达莫联合使用可能对癌症化疗有用。
