Romesser J A, O'Keefe D P
Biochem Biophys Res Commun. 1986 Oct 30;140(2):650-9. doi: 10.1016/0006-291x(86)90781-3.
Inducible cometabolism of several sulfonylurea herbicides by Streptomyces griseolus has been shown to occur by hydroxylation, O-dealkylation, or deesterification reactions. Only after growth of the bacterium in the presence of sulfonylurea did cell-free extracts exhibit NAD(P)H-dependent sulfonylurea metabolism. These extracts were shown to contain elevated levels of soluble cytochrome P-450 and exhibit sulfonylurea induced difference spectra consistent with binding of substrate to cytochrome(s) P-450. These results establish the presence of an inducible cytochrome P-450-dependent sulfonylurea metabolizing system in S. griseolus.
灰色链霉菌对几种磺酰脲类除草剂的诱导共代谢已被证明是通过羟基化、O-脱烷基化或脱酯反应发生的。只有在细菌在磺酰脲存在下生长后，无细胞提取物才表现出依赖NAD(P)H的磺酰脲代谢。这些提取物显示含有升高水平的可溶性细胞色素P-450，并表现出与底物与细胞色素P-450结合一致的磺酰脲诱导差异光谱。这些结果证实了灰色链霉菌中存在一种可诱导的细胞色素P-450依赖性磺酰脲代谢系统。
