表达大鼠细胞色素P4501A1（CYP1A1）与酵母NADPH-细胞色素P450氧化还原酶融合酶的抗除草剂烟草植株。
Herbicide-resistant tobacco plants expressing the fused enzyme between rat cytochrome P4501A1 (CYP1A1) and yeast NADPH-cytochrome P450 oxidoreductase.
作者信息
Shiota N, Nagasawa A, Sakaki T, Yabusaki Y, Ohkawa H
机构信息
Graduate School of Science and Technology, Kobe University, Japan.
出版信息
Plant Physiol. 1994 Sep;106(1):17-23. doi: 10.1104/pp.106.1.17.
Abstract
Transgenic tobacco (Nicotiana tabacum cv Xanthi) plants expressing a genetically engineered fused enzyme between rat cytochrome P4501A1 (CYP1A1) and yeast NADPH-cytochrome P450 oxidoreductase were produced. The expression plasmid pGFC2 for the fused enzyme was constructed by insertion of the corresponding cDNA into the expression vector pNG01 under the control of the cauliflower mosaic virus 35S promoter and nopaline synthase gene terminator. The fused enzyme cDNA was integrated into tobacco genomes by Agrobacterium infection techniques. In transgenic tobacco plants, the fused enzyme protein was localized primarily in the microsomal fraction. The microsomal monooxygenase activities were approximately 10 times higher toward both 7-ethoxycoumarin and benzo[a]pyrene than in the control plant. The transgenic plants also showed resistance to the herbicide chlortoluron.
摘要
培育出了表达大鼠细胞色素P4501A1（CYP1A1）与酵母NADPH - 细胞色素P450氧化还原酶之间基因工程融合酶的转基因烟草（烟草品种Xanthi）植株。通过将相应的cDNA插入到花椰菜花叶病毒35S启动子和胭脂碱合酶基因终止子控制下的表达载体pNG01中，构建了融合酶的表达质粒pGFC2。利用农杆菌感染技术将融合酶cDNA整合到烟草基因组中。在转基因烟草植株中，融合酶蛋白主要定位于微粒体部分。微粒体单加氧酶对7 - 乙氧基香豆素和苯并[a]芘的活性比对照植株高约10倍。转基因植株对除草剂绿麦隆也表现出抗性。
相似文献
1
Herbicide-resistant tobacco plants expressing the fused enzyme between rat cytochrome P4501A1 (CYP1A1) and yeast NADPH-cytochrome P450 oxidoreductase.表达大鼠细胞色素P4501A1（CYP1A1）与酵母NADPH-细胞色素P450氧化还原酶融合酶的抗除草剂烟草植株。
Plant Physiol. 1994 Sep;106(1):17-23. doi: 10.1104/pp.106.1.17.
2
Expression of human cytochromes P450 1A1 and P450 1A2 as fused enzymes with yeast NADPH-cytochrome P450 oxidoreductase in transgenic tobacco plants.人细胞色素P450 1A1和P450 1A2与酵母NADPH-细胞色素P450氧化还原酶融合酶在转基因烟草植株中的表达
Biosci Biotechnol Biochem. 2000 Oct;64(10):2025-33. doi: 10.1271/bbb.64.2025.
3
A genetically engineered P450 monooxygenase: construction of the functional fused enzyme between rat cytochrome P450c and NADPH-cytochrome P450 reductase.一种基因工程改造的细胞色素P450单加氧酶：大鼠细胞色素P450c与NADPH-细胞色素P450还原酶之间功能性融合酶的构建。
DNA. 1987 Jun;6(3):189-97. doi: 10.1089/dna.1987.6.189.
4
Functional expression of fused enzymes between human cytochrome P4501A1 and human NADPH-cytochrome P450 oxidoreductase in Saccharomyces cerevisiae.人细胞色素P4501A1与人NADPH-细胞色素P450氧化还原酶融合酶在酿酒酵母中的功能表达。
DNA Cell Biol. 1995 Apr;14(4):273-83. doi: 10.1089/dna.1995.14.273.
5
Molecular cloning and expression in Saccharomyces cerevisiae of tobacco NADPH-cytochrome P450 oxidoreductase cDNA.烟草NADPH-细胞色素P450氧化还原酶cDNA的分子克隆及其在酿酒酵母中的表达
Biosci Biotechnol Biochem. 1998 Jul;62(7):1403-11. doi: 10.1271/bbb.62.1403.
6
Genetically engineered modification of P450 monooxygenases: functional analysis of the amino-terminal hydrophobic region and hinge region of the P450/reductase fused enzyme.细胞色素P450单加氧酶的基因工程改造：P450/还原酶融合酶氨基末端疏水区域和铰链区域的功能分析
DNA. 1988 Dec;7(10):701-11. doi: 10.1089/dna.1988.7.701.
7
Coexpression of genetically engineered fused enzyme between yeast NADPH-P450 reductase and human cytochrome P450 3A4 and human cytochrome b5 in yeast.酵母中基因工程改造的酵母NADPH-P450还原酶与人细胞色素P450 3A4及人细胞色素b5之间的融合酶的共表达。
Arch Biochem Biophys. 2000 Sep 1;381(1):164-70. doi: 10.1006/abbi.2000.1953.
8
Functional expression of Saccharomyces cerevisiae CYP51A1 encoding lanosterol-14-demethylase in tobacco results in bypass of endogenous sterol biosynthetic pathway and resistance to an obtusifoliol-14-demethylase herbicide inhibitor.编码羊毛甾醇-14-去甲基酶的酿酒酵母CYP51A1在烟草中的功能表达导致内源性甾醇生物合成途径的旁路形成，并对一种钝叶醇-14-去甲基酶除草剂抑制剂产生抗性。
Plant J. 1995 May;7(5):761-70. doi: 10.1046/j.1365-313x.1995.07050761.x.
9
Establishment of a novel host, high-red yeast that stably expresses hamster NADPH-cytochrome P450 oxidoreductase: usefulness for examination of the function of mammalian cytochrome P450.一种稳定表达仓鼠NADPH-细胞色素P450氧化还原酶的新型宿主——高产红酵母的构建：对研究哺乳动物细胞色素P450功能的实用性。
Arch Biochem Biophys. 1997 Jul 15;343(2):215-24. doi: 10.1006/abbi.1997.0148.
10
Engineering herbicide metabolism in tobacco and Arabidopsis with CYP76B1, a cytochrome P450 enzyme from Jerusalem artichoke.利用来自菊芋的细胞色素P450酶CYP76B1在烟草和拟南芥中构建除草剂代谢工程。
Plant Physiol. 2002 Sep;130(1):179-89. doi: 10.1104/pp.005801.
引用本文的文献
1
Enhancement of naphthalene tolerance in transgenic Arabidopsis plants overexpressing the ferredoxin-like protein (ADI1) from rice.过表达来自水稻的铁氧还蛋白样蛋白（ADI1）的转基因拟南芥植株中萘耐受性的增强。
Plant Cell Rep. 2016 Jan;35(1):17-26. doi: 10.1007/s00299-015-1861-2. Epub 2015 Nov 18.
2
Engineering the metabolism of the phenylurea herbicide chlortoluron in genetically modified Arabidopsis thaliana plants expressing the mammalian cytochrome P450 enzyme CYP1A2.在表达哺乳动物细胞色素 P450 酶 CYP1A2 的转基因拟南芥植物中工程化苯脲类除草剂绿麦隆的代谢。
Environ Sci Pollut Res Int. 2014;21(13):8224-32. doi: 10.1007/s11356-014-2710-5. Epub 2014 Mar 29.
3
A simple shoot multiplication procedure using internode explants, and its application for particle bombardment and Agrobacterium-mediated transformation in watercress.利用节间外植体进行简单的芽增殖程序及其在西洋菜中的粒子轰击和农杆菌介导转化中的应用。
J Plant Res. 2009 Jul;122(4):455-63. doi: 10.1007/s10265-009-0226-8. Epub 2009 Mar 24.
4
Analysis of substrate specificity of pig CYP2B22 and CYP2C49 towards herbicides by transgenic rice plants.利用转基因水稻植株分析猪CYP2B22和CYP2C49对除草剂的底物特异性。
Transgenic Res. 2005 Dec;14(6):907-17. doi: 10.1007/s11248-005-0199-x.
5
Identification and analysis of safener-inducible expressed sequence tags in Populus using a cDNA microarray.利用cDNA微阵列鉴定和分析杨树中安全剂诱导表达的序列标签
Planta. 2004 Dec;220(2):296-306. doi: 10.1007/s00425-004-1356-9. Epub 2004 Sep 18.
6
Engineering herbicide metabolism in tobacco and Arabidopsis with CYP76B1, a cytochrome P450 enzyme from Jerusalem artichoke.利用来自菊芋的细胞色素P450酶CYP76B1在烟草和拟南芥中构建除草剂代谢工程。
Plant Physiol. 2002 Sep;130(1):179-89. doi: 10.1104/pp.005801.
7
Enhanced metabolism of halogenated hydrocarbons in transgenic plants containing mammalian cytochrome P450 2E1.含哺乳动物细胞色素P450 2E1的转基因植物中卤代烃代谢增强。
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6287-91. doi: 10.1073/pnas.97.12.6287.
8
Expression of a soybean cytochrome P450 monooxygenase cDNA in yeast and tobacco enhances the metabolism of phenylurea herbicides.大豆细胞色素P450单加氧酶cDNA在酵母和烟草中的表达增强了苯基脲类除草剂的代谢。
Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1750-5. doi: 10.1073/pnas.96.4.1750.
9
The chemically inducible plant cytochrome P450 CYP76B1 actively metabolizes phenylureas and other xenobiotics.化学诱导型植物细胞色素P450 CYP76B1可积极代谢苯基脲和其他异生素。
Plant Physiol. 1998 Nov;118(3):1049-56. doi: 10.1104/pp.118.3.1049.
本文引用的文献
1
Engineering herbicide resistance in plants by expression of a detoxifying enzyme.通过表达解毒酶来工程植物的抗除草剂性。
EMBO J. 1987 Sep;6(9):2513-8. doi: 10.1002/j.1460-2075.1987.tb02537.x.
2
Purification, characterization, and cDNA cloning of an NADPH-cytochrome P450 reductase from mung bean.绿豆中NADPH-细胞色素P450还原酶的纯化、特性鉴定及cDNA克隆
Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2890-4. doi: 10.1073/pnas.90.7.2890.
3
Kinetic studies on a genetically engineered fused enzyme between rat cytochrome P4501A1 and yeast NADPH-P450 reductase.关于大鼠细胞色素P4501A1与酵母NADPH-P450还原酶之间基因工程融合酶的动力学研究。
Biochemistry. 1994 Apr 26;33(16):4933-9. doi: 10.1021/bi00182a023.
4
The P450 superfamily: update on new sequences, gene mapping, accession numbers, early trivial names of enzymes, and nomenclature.细胞色素P450超家族：新序列、基因图谱、登录号、酶的早期俗名及命名法的更新
DNA Cell Biol. 1993 Jan-Feb;12(1):1-51. doi: 10.1089/dna.1993.12.1.
5
Characterization of rat cytochrome P-450MC synthesized in Saccharomyces cerevisiae.在酿酒酵母中合成的大鼠细胞色素P-450MC的特性分析。
J Biochem. 1985 Jul;98(1):167-75. doi: 10.1093/oxfordjournals.jbchem.a135255.
6
Induction of cytochrome P-450-dependent sulfonylurea metabolism in Streptomyces griseolus.灰色链霉菌中细胞色素P-450依赖性磺酰脲代谢的诱导。
Biochem Biophys Res Commun. 1986 Oct 30;140(2):650-9. doi: 10.1016/0006-291x(86)90781-3.
7
Expression in Saccharomyces cerevisiae of chimeric cytochrome P450 cDNAs constructed from cDNAs for rat cytochrome P450c and P450d.由大鼠细胞色素P450c和P450d的cDNA构建的嵌合细胞色素P450 cDNA在酿酒酵母中的表达。
DNA. 1987 Feb;6(1):31-9. doi: 10.1089/dna.1987.6.31.
8
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.GUS融合：β-葡萄糖醛酸酶作为高等植物中一种灵敏且通用的基因融合标记
EMBO J. 1987 Dec 20;6(13):3901-7. doi: 10.1002/j.1460-2075.1987.tb02730.x.
9
A genetically engineered P450 monooxygenase: construction of the functional fused enzyme between rat cytochrome P450c and NADPH-cytochrome P450 reductase.一种基因工程改造的细胞色素P450单加氧酶：大鼠细胞色素P450c与NADPH-细胞色素P450还原酶之间功能性融合酶的构建。
DNA. 1987 Jun;6(3):189-97. doi: 10.1089/dna.1987.6.189.
10
Expression of cloned yeast NADPH-cytochrome P450 reductase gene in Saccharomyces cerevisiae.克隆的酵母NADPH-细胞色素P450还原酶基因在酿酒酵母中的表达。
J Biochem. 1990 Nov;108(5):859-65. doi: 10.1093/oxfordjournals.jbchem.a123293.
Zotero 插件