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高等植物微粒体中一种细胞色素P-450单加氧酶对 obtusifoliol 的14α-去甲基化作用。

The 14 alpha-demethylation of obtusifoliol by a cytochrome P-450 monooxygenase from higher plants' microsomes.

作者信息

Rahier A, Taton M

出版信息

Biochem Biophys Res Commun. 1986 Nov 14;140(3):1064-72. doi: 10.1016/0006-291x(86)90743-6.

DOI:10.1016/0006-291x(86)90743-6
PMID:3778482
Abstract

Microsomes isolated from corn embryos (Zea mays) can demethylate the 14 alpha-methyl group of obtusifoliol 2. An enzymatic assay has been developed for obtusifoliol 14 alpha-methyl-demethylase in higher plants. The enzymatic reaction was shown to occur sequentially, converting obtusifoliol 2 to 4 alpha-methyl-5 alpha-ergosta-8,24(28)-dien-3 beta-ol 4 via the trienol 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol 3 which was thoroughly identified. This enzymatic reaction is dependent of NADPH and molecular oxygen. It is inhibited by CO, menadione and specific inhibitors of cytochrome P-450, the CO inhibition being partially reversed by light. It is concluded that in Zea mays microsomes, obtusifoliol is demethylated at C-14 by a cytochrome P-450 containing monooxygenase system.

摘要

从玉米胚（玉米）中分离出的微粒体能够使钝叶醇2的14α-甲基去甲基化。已经开发出一种用于高等植物中钝叶醇14α-甲基去甲基化酶的酶促测定法。酶促反应显示为顺序发生，通过已被充分鉴定的三烯醇4α-甲基-5α-麦角甾-8,14,24(28)-三烯-3β-醇3将钝叶醇2转化为4α-甲基-5α-麦角甾-8,24(28)-二烯-3β-醇4。这种酶促反应依赖于NADPH和分子氧。它受到CO、甲萘醌和细胞色素P-450的特异性抑制剂的抑制，CO抑制作用可被光部分逆转。得出的结论是，在玉米微粒体中，钝叶醇在C-14位通过含细胞色素P-450的单加氧酶系统去甲基化。

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