Miller F R, McInerney D, Miller B E
Biochem Biophys Res Commun. 1986 Nov 14;140(3):955-61. doi: 10.1016/0006-291x(86)90728-x.
We utilized two assays for metabolic cooperation in vitro, one in which cells were grown in monolayer and one in which the cells formed three-dimensional structures in a collagen gel matrix. Both assays required one of the test cell pair to be resistant both to ouabain and to thioguanine (deficient in hypoxanthine-guanine phosphoribosyltransferase). Normal mammary gland cells, cells from preneoplastic mouse hyperplastic alveolar nodules, and mouse mammary tumor cells were metabolically linked in vitro to the drug-resistant tumor cells. Ouabain abrogated communication between tumor cells and normal mammary gland cells and between tumor cells and preneoplastic cells but had no effect on communication between tumor cells.
我们使用了两种体外代谢协作检测方法，一种是让细胞在单层培养，另一种是让细胞在胶原蛋白凝胶基质中形成三维结构。两种检测方法都要求测试细胞对哇巴因和硫鸟嘌呤具有抗性（次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶缺乏）。正常乳腺细胞、来自癌前小鼠增生性肺泡结节的细胞以及小鼠乳腺肿瘤细胞在体外与耐药肿瘤细胞存在代谢联系。哇巴因消除了肿瘤细胞与正常乳腺细胞之间以及肿瘤细胞与癌前细胞之间的通讯，但对肿瘤细胞之间的通讯没有影响。
