Iriyama K, Iwamoto T, Yoshiura M, Aoki T
Biochem Med Metab Biol. 1986 Oct;36(2):186-93. doi: 10.1016/0885-4505(86)90123-4.
There has been no report on the determination of uric acid (UA) in human brain and heart tissues. UA and ascorbic acid (AA) in human cerebral cortex and heart tissues excised after cardiac death have been studied by reversed-phase high-performance liquid chromatography (HPLC) with electrochemical detection (ECD). It has been found that the levels of AA and UA in the human cerebral cortex tissues tend to decrease and increase, respectively, after cardiac death as a function of time between death and forensic operation. In addition, it has been found that there is no special relationship between UA levels in human heart tissues and time after cardiac death, also that the UA levels in the heart are high as compared with those in human cerebral cortex tissues. We have emphasized that the HPLC-ECD method is useful in determining UA and AA in mammalian tissues by one-time chromatography to gain a better understanding of the relationship between disease and serum urate level.
目前尚无关于人脑组织和心脏组织中尿酸（UA）测定的报道。采用反相高效液相色谱（HPLC）结合电化学检测（ECD）法，对心源性死亡后切除的人大脑皮质和心脏组织中的UA和抗坏血酸（AA）进行了研究。研究发现，人大脑皮质组织中的AA和UA水平在心源性死亡后，会随着死亡与法医解剖之间的时间变化而分别呈现下降和上升的趋势。此外，还发现人心脏组织中的UA水平与心源性死亡后的时间并无特殊关系，而且心脏中的UA水平高于人大脑皮质组织中的UA水平。我们强调，HPLC-ECD方法可通过一次色谱分析来测定哺乳动物组织中的UA和AA，从而更好地了解疾病与血清尿酸水平之间的关系。
