BACKGROUND

Hypoxic-ischemic encephalopathy (HIE) could induce exacerbated changes and unpredictable effects in brain cells, and the mechanism remains unclear.

METHODS

HIE model was established in neonatal rats, Zea-Longa score and TTC staining were used to observe the neurobehavior and brain infarct volume in rats subjected to cerebral hypoxia-ischemia (HI). Primary cortical neurons were then cultured to establish an oxygen and glucose deprivation model. To determine the role of synaptosomal-associated protein-25 () in HIE, PC12 cells were cultured and effective siRNA fragments were screened, and was transfected into primary neurons. Then, quantitative real-time polymerase chain reaction was used to detect the mRNA expression level and immunofluorescence staining was used to observe the morphological changes of neurons before and after the injury. Finally, the abundance values of and its associated genes were filtered using the NCBI and GeneMANIA, respectively.

RESULTS

HI leads to a decrease in neuronal number and an increase in expression. Whereas, the interference of caused marked decrease in neuronal number and the length of neurite. Moreover, the expression levels of CREB and SYP were significantly decreased after interference of .

CONCLUSION

exhibited several neuroprotective effects to neuronal protection in neonatal cerebral HI by regulating CREB and SYP.