Li Juan, Pan Yuan-Tao, Chen Jun-Jie, Wang Yi, Zhou Hong-Su, Huang Xue-Yan, Liao Shi-Xia
Department of Anesthesiology West China Hospital, Sichuan University Chengdu Sichuan China.
Department of Anesthesiology Affiliated Hospital of Zunyi Medical University Zunyi Guizhou China.
Ibrain. 2021 Jun 28;7(2):95-107. doi: 10.1002/j.2769-2795.2021.tb00071.x. eCollection 2021 Jun.
Explore the relationship between the neural function deficit and the changes of lncRNA and mRNA in hippocampus after traumatic brain injury (TBI) in rats.
Twenty male rats weighted 200-240 grams were randomly divided into sham group and TBI group. Neurologic severity score (NSS) was performed after operation, and the hippocampus of rats was collected for long non-coding RNAs (lncRNAs), mRNAs microarray detection, real-time quantitative PCR Detecting System (Q-PCR), western blot (WB) detection, and serum biochemical detection.
The NSS score of the TBI group was significantly higher than the sham group. Compared with the sham group, 270 lncRNAs changed in the TBI group, of which 224 were up-regulated and 46 were down-regulated. Among up-regulated lncRNAs, mRNAs were distributed in upstream of 22 lncRNAs, downstream of 17 lncRNAs, overlapping regions of 48 lncRNAs, and antisense chains of 21 lncRNAs. Among down-regulated lncRNAs, mRNAs were distributed in upstream of 6 lncRNAs, downstream of 3 lncRNAs, overlapping regions of 10 lncRNAs, and antisense chains of 8 lncRNAs. Compared with the sham group, 1054 mRNA changed in the TBI group, of which 921 mRNA were up-regulated and 133 mRNA were down-regulated. The expression changes of ENSRNOT000063054, ENSRNOT000052790, ENSRNOT00000054410, ENSRNOT000063242, and ENSRNOT000069411 IncRNA regulate the expression of Top2a, RT1-CE11, Papss2, Stk32a, and Grid2 gene.
The present study detected the differential expression of lncRNAs and mRNAs in hippocampi of rats subjected to TBI, and discussed their relation, primarily.
探讨大鼠创伤性脑损伤（TBI）后神经功能缺损与海马区lncRNA和mRNA变化之间的关系。
将20只体重200 - 240克的雄性大鼠随机分为假手术组和TBI组。术后进行神经功能严重程度评分（NSS），并采集大鼠海马组织进行长链非编码RNA（lncRNAs）、mRNA芯片检测、实时定量PCR检测系统（Q-PCR）、蛋白质免疫印迹（WB）检测以及血清生化检测。
TBI组的NSS评分显著高于假手术组。与假手术组相比，TBI组中有270个lncRNAs发生变化，其中224个上调，46个下调。在上调的lncRNAs中，mRNAs分布于22个lncRNAs的上游、17个lncRNAs的下游、48个lncRNAs的重叠区域以及21个lncRNAs的反义链。在下调的lncRNAs中，mRNAs分布于6个lncRNAs的上游、3个lncRNAs的下游、10个lncRNAs的重叠区域以及8个lncRNAs的反义链。与假手术组相比，TBI组中有1054个mRNA发生变化，其中921个mRNA上调，133个mRNA下调。ENSRNOT000063054、ENSRNOT000052790、ENSRNOT00000054410、ENSRNOT000063242和ENSRNOT000069411 IncRNA的表达变化调节Top2a、RT1 - CE11、Papss2、Stk32a和Grid2基因的表达。
本研究检测了TBI大鼠海马中lncRNAs和mRNAs的差异表达，并初步探讨了它们之间的关系。
