Herrmann T R, Jayaweera A R, Shamoo A E
Biochemistry. 1986 Sep 23;25(19):5834-8. doi: 10.1021/bi00367a074.
The technique of laser-excited Eu(III) luminescence was applied to monitor Eu(III) binding to a variety of phospholipids. Eu(III) excitation spectra were similar with and without the presence of neutral phospholipids, while acidic phospholipids changed the spectrum in a concentration-dependent manner. Eu(III) appears to bind to the phosphate moiety with at least a 2:1 phospholipid:metal ion stoichiometry. Analysis of luminescence lifetimes reveals that only one or two waters of hydration are removed from Eu(III) by addition of neutral phospholipids, whereas acidic phospholipids and inorganic phosphate strip off all but one or two waters. Implications with regard to fusion and use of lanthanides as probes in membrane preparations are discussed.
激光激发铕（III）发光技术被用于监测铕（III）与多种磷脂的结合。有无中性磷脂存在时，铕（III）的激发光谱相似，而酸性磷脂则以浓度依赖的方式改变光谱。铕（III）似乎以至少2:1的磷脂:金属离子化学计量比与磷酸基团结合。发光寿命分析表明，添加中性磷脂时，铕（III）仅去除一两个水化水分子，而酸性磷脂和无机磷酸盐则去除除一两个水分子外的所有水分子。文中讨论了有关镧系元素在膜制剂中作为融合和探针的应用。
