Hubbell J A, McIntire L V
Biomaterials. 1986 Sep;7(5):354-63. doi: 10.1016/0142-9612(86)90006-2.
Visualization and analysis of mural thrombogenesis on collagen-coated glass, polyurethane and nylon was discussed. Epi-fluorescent video microscopy was used to visualize thrombotic events at a protein or polymer surface in contact with flowing whole blood. Digital image processing was used to analyse the real-time microscopic images obtained, resulting in measurements of morphological features and the number of platelets that compose each thrombus. The three-dimensional structures of the thrombi were also estimated. A photodiode was used to measure integrated end-point platelet accumulation at different axial positions along the surface. Additionally, the convection-diffusion equations were solved to estimate the concentrations of adenosine diphosphate, thromboxane A2, and thrombin generated by activated platelets at the blood-contacting surface.
讨论了在胶原包被的玻璃、聚氨酯和尼龙上对壁血栓形成的可视化和分析。落射荧光视频显微镜用于观察与流动全血接触的蛋白质或聚合物表面的血栓形成事件。利用数字图像处理分析所获得的实时显微图像，从而测量组成每个血栓的形态特征和血小板数量。还估计了血栓的三维结构。使用光电二极管测量沿表面不同轴向位置的积分终点血小板聚集。此外，求解对流扩散方程以估计由活化血小板在血液接触表面产生的二磷酸腺苷、血栓素A2和凝血酶的浓度。
