对从健康人群、绵羊乳腺炎和奶酪中分离出的菌株的一些毒力因子进行检测。

The examination of some virulence factors in isolates obtained from the healthy human population, sheep mastitis, and cheese.

作者信息

Rahbarnia L, Khosravi Rad R, Dehnad A R, Naghili B

机构信息

Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

MSc in Microbiology, Higher Education Institute of Rab-Rashid, Tabriz, Iran.

出版信息

Iran J Vet Res. 2023;24(2):110-115. doi: 10.22099/IJVR.2023.43730.6410.

DOI:10.22099/IJVR.2023.43730.6410

PMID:37790118

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10542877/

Abstract

UNLABELLED

Abstract.

BACKGROUND

Staphylococcus aureus is responsible for many infections in humans and animals from skin and soft tissue infections to life-threatening diseases. In this study to explore the origin of S. aureus infections in humans, the antibiotic resistance profile and the variety of virulence factors in S. aureus isolates were examined in three groups: a healthy human population, cheese, and the milk of sheep with mastitis.

AIMS

The examination of some virulence factors in isolates obtained from the healthy human population, sheep mastitis, and cheese.

METHODS

A total of 400 nasal swab samples from healthy students, 30 cheese samples, and 122 sheep milk samples were collected for the detection of S. aureus isolates from January 1, 2018, to March 1, 2018. The frequency of , , , , and virulence genes and mecA gene was determined in each group by PCR assay.

RESULTS

There was a direct relationship between the antibiotic susceptibility profile of the isolates from a healthy population and those from mastitis milk samples. Of 400 nasal samples, 15% (60/400) were positive for S. aureus of which 60% (36/60) were positive for mecA. While 50% (15/30) of cheese samples were positive for S. aureus which 7 cases (46.66%, 7/15) were positive for mecA. The prevalence of S. aureus among students was dependent on gender (P). Also, 47.5% (58/122) of milk samples from sheep mastitis were positive for , and 41.37% (24/58) were positive for the gene. Based on PCR results, the highest rate of (68.33%, 41/60), (53.33%, 32/60), and (46.66%, 28/60) genes were related to a healthy population, and the highest frequency of (41.38%, 24/58), and (27.59%, 16/58) was related to milk samples (P<0.05). A significant correlation was observed between the presence of the arginine catabolic mobile element (ACME)-arcA gene and resistance to methicillin (P<0.05)

CONCLUSION

The high rate of virulence factors in the isolates obtained from mastitis and dairy products is an alert point, because they could be source of the spreading of to humans. There is an essential need for continuous monitoring to control staphylococcal food poisoning.

摘要

未标注

摘要。

背景

金黄色葡萄球菌可导致人类和动物发生多种感染，从皮肤和软组织感染到危及生命的疾病。在本研究中，为探究人类金黄色葡萄球菌感染的来源，对三组样本中的金黄色葡萄球菌分离株的抗生素耐药谱和多种毒力因子进行了检测：健康人群、奶酪以及患有乳腺炎的绵羊的乳汁。

目的

检测从健康人群、绵羊乳腺炎和奶酪中分离得到的菌株中的一些毒力因子。

方法

在2018年1月1日至2018年3月1日期间，共收集了400份健康学生的鼻拭子样本、30份奶酪样本和122份绵羊乳汁样本，用于检测金黄色葡萄球菌分离株。通过聚合酶链反应（PCR）测定每组中、、、和毒力基因以及mecA基因的频率。

结果

健康人群分离株与乳腺炎乳汁样本分离株的抗生素敏感性谱之间存在直接关系。在400份鼻拭子样本中，15%（60/400）金黄色葡萄球菌呈阳性，其中60%（36/60）mecA呈阳性。而30份奶酪样本中有50%（15/30）金黄色葡萄球菌呈阳性，其中7份（46.66%，7/15）mecA呈阳性。学生中金黄色葡萄球菌的患病率取决于性别（P）。此外，47.5%（58/122）患有乳腺炎的绵羊乳汁样本呈阳性，41.37%（24/58）基因呈阳性。基于PCR结果，（68.33%，41/60）、（53.33%，32/60）和（46.66%，28/60）基因的最高检出率与健康人群相关，（41.38%，24/58）和（27.59%，16/58）的最高频率与乳汁样本相关（P<0.05）。观察到精氨酸分解代谢移动元件（ACME）-arcA基因与对甲氧西林耐药之间存在显著相关性（P<0.05）。