Sheikhzadeh Behnood Haji, Rahbarnia Leila, Dehnad Alireza, Naghili Behrooz, Saffarian Parvaneh
Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Iran J Microbiol. 2023 Jun;15(3):408-413. doi: 10.18502/ijm.v15i3.12901.
Methicillin-resistant (MRSA) is one of the main causes of high mortality and morbidity in hospitals. This study was aimed to examine virulence factors, molecular typing, and the antibiotic resistance pattern of MRSA isolates in hemodialysis patients and healthy communities.
Total of 231 and 400 nasal samples were obtained from hemodialysis patients and healthy communities, respectively. Virulence factors profile was examined in two groups by PCR reaction. Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) was used as a molecular typing approach.
Overall, 35.49% (82/231) of hemodialysis patients were positive for and 47.56% (39/82) of isolates were positive for . In a healthy community, 15% (60/400) of samples were positive for , and 36.66% (22/60) were positive for . The frequency of MDR was significantly higher in patients group (p-value < 0.00001). The frequency of (p.value = 0.003932, P<0.05) and (p.value = 0.003173, p < .05) were significantly higher in patients group. The highest frequency virulence factors in healthy individuals were related to (68.33%, 41/60), (53.33%, 32/60), and (46.66%) genes. Two groups were clustered by the ERIC-PCR method into 7 clusters and 2 single isolate with a 0.74 similarity index. Based on the results, each cluster was combination with healthy and patient isolates.
Our findings indicate a notable variation in the frequency of virulence factors between isolates obtained from dialysis patients and the healthy community.
耐甲氧西林金黄色葡萄球菌（MRSA）是医院高死亡率和高发病率的主要原因之一。本研究旨在检测血液透析患者和健康社区中MRSA分离株的毒力因子、分子分型及抗生素耐药模式。
分别从血液透析患者和健康社区获取了231份和400份鼻拭子样本。通过PCR反应检测两组的毒力因子谱。采用肠杆菌重复基因间共识序列（ERIC-PCR）作为分子分型方法。
总体而言，35.49%（82/231）的血液透析患者MRSA检测呈阳性，其中47.56%（39/82）的分离株检测呈阳性。在健康社区中，15%（60/400）的样本MRSA检测呈阳性，其中36.66%（22/60）检测呈阳性。患者组中多重耐药（MDR）的频率显著更高（p值<0.00001）。患者组中 （p值=0.003932，P<0.05）和 （p值=0.003173，p<0.05）的频率显著更高。健康个体中毒力因子频率最高的与 （68.33%，41/60）、 （53.33%，32/60）和 （46.66%）基因相关。通过ERIC-PCR方法将两组分为7个簇和2个单分离株，相似性指数为0.74。根据结果，每个簇均由健康和患者分离株组成。
我们的研究结果表明，从透析患者和健康社区获得的MRSA分离株之间，毒力因子频率存在显著差异。
