Fleming Derek, Bozyel Ibrahim, Ozdemir Dilara, Otero Judith Alvarez, Karau Melissa J, Islam Anoy Md Monzurul, Koscianski Christina, Schuetz Audrey N, Greenwood-Quaintance Kerryl E, Mandrekar Jayawant N, Beyenal Haluk, Patel Robin
bioRxiv. 2023 Sep 21:2023.09.20.558698. doi: 10.1101/2023.09.20.558698.
A novel electrochemical bandage (e-bandage) delivering low-level hypochlorous acid (HOCl) was evaluated against murine wound biofilms. 5 mm skin wounds were created on the dorsum of Swiss-Webster mice and infected with 10 colony forming units (CFU) of . Biofilms were formed over two days, after which e-bandages were placed on the wound beds and covered with Tegaderm™. Mice were administered Tegaderm-only (control), non-polarized e-bandage (no HOCl production), or polarized e-bandage (using an HOCl-producing potentiostat), with or without concurrently administered systemic amikacin. Purulence and wound areas were measured before and after treatment. After 48 hours, animals were sacrificed, and wounds were harvested for bacterial quantification. Forty-eight hours of polarized e-bandage treatment resulted in mean biofilm reductions of 1.4 log CFUs/g (9.0 vs 7.6 log ; p = 0.0107) non-polarized controls, and 2.2 log CFU/g (9.8 vs 7.6 log ; p = 0.004) Tegaderm only controls. Systemic amikacin improved CFU reduction in Tegaderm-only (p = 0.0045) and non-polarized control groups (p = 0.0312), but not in the polarized group (p = 0.3876). Compared to the Tegaderm only group, there was more purulence reduction in the polarized group (p = 0.009), but not in the non-polarized group (p = 0.064). Wound closure was not impeded or improved by either polarized or non-polarized e-bandage treatment. Concurrent amikacin did not impact wound closure or purulence. In conclusion, an HOCl-producing e-bandage reduced in wound biofilms with no impairment in wound healing, representing a promising antibiotic-free approach for addressing wound infections.
一种新型的能释放低浓度次氯酸（HOCl）的电化学绷带（电子绷带）针对小鼠伤口生物膜进行了评估。在瑞士 Webster 小鼠的背部制造 5 毫米的皮肤伤口，并接种 10 个菌落形成单位（CFU）的[具体细菌名称未给出]。生物膜在两天内形成，之后将电子绷带放置在伤口床上，并用 Tegaderm™覆盖。给小鼠使用仅 Tegaderm（对照）、非极化电子绷带（不产生 HOCl）或极化电子绷带（使用产生 HOCl 的恒电位仪），同时或不同时给予全身性阿米卡星。在治疗前后测量脓性分泌物和伤口面积。48 小时后，处死动物，收集伤口进行细菌定量分析。48 小时的极化电子绷带治疗使生物膜平均减少 1.4 个对数 CFU/g（9.0 对 7.6 对数；p = 0.0107），相对于非极化对照组，以及 2.2 个对数 CFU/g（9.8 对 7.6 对数；p = 0.004）相对于仅 Tegaderm 对照组。全身性阿米卡星改善了仅 Tegaderm 组（p = 0.0045）和非极化对照组（p = 0.0312）的 CFU 减少情况，但在极化组未改善（p = 0.3876）。与仅 Tegaderm 组相比，极化组脓性分泌物减少更多（p = 0.009），但非极化组未减少（p = 0.064）。极化或非极化电子绷带治疗均未阻碍或改善伤口愈合。同时使用阿米卡星不影响伤口愈合或脓性分泌物情况。总之，一种产生 HOCl 的电子绷带可减少伤口生物膜中的[细菌名称未给出]，且不损害伤口愈合，代表了一种有前景的无抗生素方法来解决伤口感染问题。
