The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA; Department of Electrical and Computer Engineering, Worcester Polytechnic Institute, Worcester, MA, USA.
Division of Clinical Microbiology, Mayo Clinic, Rochester, MN, USA.
Biosens Bioelectron. 2025 Jan 1;267:116791. doi: 10.1016/j.bios.2024.116791. Epub 2024 Sep 24.
We have developed electrochemical bandage (e-bandage) prototypes that generate the reactive oxygen species hypochlorous acid (HOCl) or hydrogen peroxide (HO) for potential use to treat biofilm-infected wounds in humans. We have shown that both e-bandage-generated HOCl and HO kill biofilms in vitro and in infected wounds on mice, with the former being more active in vitro. The HO-generating e-bandage, more so than the HOCl-generating e-bandage, was associated with improved healing of infected wounds. Here, a strategy in which HO and HOCl are alternately generated-for dual action-was explored. The goal was to develop a programmable multimodal wearable potentiostat [PMWP] that can generate HOCl or HO, as needed. An ultralow-power microcontroller unit was developed to manage operation of the PMWP. The system was operated with a 260-mAh capacity coin battery and weighed 4.6 g, making it suitable for future small animal experiments (and ultimately, potential evaluation in humans). As assessed using electrochemical parameters, the device functioned comparably to a commercial benchtop potentiostat. To confirm antimicrobial activity, PMWP-controlled e-bandages were tested in vitro against clinical isolates of methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, Enterococcus faecium, and Candida auris. When programmed to deliver HOCl followed by HO, PMWP-controlled e-bandages exhibited activity against biofilms of all study isolates tested. Finally, we demonstrated the PMWP's usability in a murine wound infection model.
我们开发了电化学绷带（e-bandage）原型，可生成次氯酸（HOCl）或过氧化氢（HO）等活性氧物质，有望用于治疗人类生物膜感染伤口。我们已经证明，e-bandage 生成的 HOCl 和 HO 均可在体外和感染小鼠的伤口中杀死生物膜，前者在体外更具活性。与生成 HOCl 的 e-bandage 相比，生成 HO 的 e-bandage 更有助于感染伤口的愈合。在这里，我们探索了一种交替生成 HO 和 HOCl 的策略——双效作用。目的是开发一种可编程的多模态可穿戴式电势计 [PMWP]，可根据需要生成 HOCl 或 HO。我们开发了一个超低功耗微控制器单元来管理 PMWP 的操作。该系统使用 260mAh 容量的硬币电池运行，重量为 4.6g，非常适合未来的小动物实验（最终有望在人类中进行评估）。从电化学参数评估来看，该设备的功能与商用台式电势计相当。为了确认抗菌活性，我们对 PMWP 控制的 e-bandage 进行了体外测试，以对抗耐甲氧西林金黄色葡萄球菌、铜绿假单胞菌、鲍曼不动杆菌、屎肠球菌和耳念珠菌等临床分离株。当编程为先输送 HOCl 再输送 HO 时，PMWP 控制的 e-bandage 对所有测试的研究分离株的生物膜均表现出活性。最后，我们在小鼠伤口感染模型中证明了 PMWP 的可用性。
