Department of Chemistry, Tianjin Key Laboratory of Molecular Optoelectronic Sciences School of Science, Tianjin University, Tianjin 300072, China.
Tianjin Key Laboratory of Drug Targeting and Bioimaging, Life and Health Intelligent Research Insitute, Tianjin University of Technology, Tianjin 300384, China.
Nanoscale. 2023 Oct 20;15(40):16458-16465. doi: 10.1039/d3nr03405d.
Organic field effect transistors have emerged as promising platforms for biosensing applications. However, the challenge lies in optimizing functionalization strategies for the sensing interface, enabling the simultaneous detection of low abundance proteins while maintaining device performance. Here, we designed a carbon dots-functionalized extended gate organic field effect transistor. Leveraging the advantages of facile synthesis, tunable modification, small particle size, and cost-effectiveness of carbon dots, we implemented their integration onto the electrode surface. Through harnessing the covalent interactions of functional groups on the surface of carbon dots, we achieved effective immobilization of low abundance proteins without compromising device performance. Consequently, this biosensor exhibits a remarkably low limit of detection of 2.7 pg mL and demonstrates high selectivity for the carcinoembryonic antigen. These findings highlight the superior capabilities of carbon dots in enhancing biosensor performance and emphasize their potential for early cancer detection.
有机场效应晶体管已成为生物传感应用的有前途的平台。然而，挑战在于优化传感界面的功能化策略，实现同时检测低丰度蛋白质，同时保持器件性能。在这里，我们设计了一种碳点功能化的扩展栅有机场效应晶体管。利用碳点易于合成、可调修饰、粒径小和成本效益高的优势，我们将其集成到电极表面。通过利用碳点表面官能团的共价相互作用，我们实现了低丰度蛋白质的有效固定，而不会影响器件性能。因此，该生物传感器的检测限低至 2.7pg/mL，对癌胚抗原具有高选择性。这些发现突出了碳点在增强生物传感器性能方面的卓越能力，并强调了它们在早期癌症检测中的潜力。
