Shen Jingxiang, Fan Zhefeng
School of Chemistry and Material Science, Shanxi Normal University, No. 339, Taiyu Road, Xiaodian District, Taiyuan, 030000, Shanxi Province, People's Republic of China.
Department of Chemistry, Changzhi University, 73 Baoningmen East Street, Changzhi, 046011, Shanxi Province, People's Republic of China.
Mikrochim Acta. 2023 Oct 4;190(11):427. doi: 10.1007/s00604-023-06005-5.
The simple preparation of a nanohybrid of terbium-doped carbon dots/glutathione-capped copper nanoclusters (Tb@CDs/GSH-CuNCs) was for the first time developed for ratiometric detection of phosphate anion (Pi). Blue-emission of Tb@CDs can trigger non-luminescence of GSH-CuNCs for aggregation-induced emission (AIE) performance due to the strong reserved coordination capacity of Tb. Thus, Tb@CDs/GSH-CuNCs rapidly generated dual-emission signals at 630 nm and 545 nm by directly mixing the two individual materials via the AIE effect, alongside fluorescence resonance energy transfer (FRET) process. However, by the introduction of Pi, both AIE and FRET processes were blocked because of the stronger binding affinity of Tb and Pi than that of Tb and -COOH on Tb@CDs, thus realizing successful ratiometric detection of Pi. The linear concentration range was 0-16 μM, with the limit of detection (LOD) of 0.32 μM. The proposed method provided new ideas for designing nanohybrid of CDs and nanoclusters (MNCs) as ratiometric fluorescent probes for analytical applications.
首次开发了一种简单的制备铽掺杂碳点/谷胱甘肽包覆铜纳米簇（Tb@CDs/GSH-CuNCs）纳米杂化物的方法，用于磷酸根阴离子（Pi）的比率检测。由于Tb具有强大的保留配位能力，Tb@CDs的蓝光发射可触发GSH-CuNCs的非发光，从而实现聚集诱导发光（AIE）性能。因此，Tb@CDs/GSH-CuNCs通过AIE效应直接混合两种单独的材料，同时伴随荧光共振能量转移（FRET）过程，在630nm和545nm处迅速产生双发射信号。然而，通过引入Pi，由于Tb与Pi的结合亲和力比Tb与Tb@CDs上的-COOH的结合亲和力更强，AIE和FRET过程均被阻断，从而实现了对Pi的成功比率检测。线性浓度范围为0-16μM，检测限（LOD）为0.32μM。该方法为设计用于分析应用的比率荧光探针的碳点和纳米簇（MNCs）纳米杂化物提供了新思路。
