Fang Xiangyang, Li Jiaxin, Cao Shidan, Mao Linmi, Cao Qiue, Liu Cui, Zhou Chuan-Hua
National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Yunnan University), School of Chemical Science and Technology, Yunnan University, Kunming, 650091, People's Republic of China.
Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, Innovative Drug Research Center, School of Pharmaceutical Sciences, Chongqing University, Chongqing, 400044, People's Republic of China.
Mikrochim Acta. 2025 Jul 8;192(8):487. doi: 10.1007/s00604-025-07350-3.
A smartphone-assisted ratiometric fluorescence sensor based on green emissive Cu nanoclusters (Cu NCs) and red emissive Ag nanoclusters (Ag NCs) is constructed, in which the Cu NCs and Ag NCs bind with each other through electrostatic interaction, leading to fluorescence resonance energy transfer from Cu NCs to Ag NCs. The copper ions (Cu) can quench the red fluorescence of the Cu NCs -Ag NCs system, while the green fluorescence remains essentially unchanged, thus enabling the fluorescence ratio detection of Cu. This method demonstrates high selectivity for Cu and a wide detection range (1.0 to 60.0 μM). The limit of detection is as low as 0.36 μM, which is significantly lower than the maximum tolerable concentration in drinking water (~ 20 μM). In addition, the fluorescence color can be converted into RGB values by a smartphone, indicating great potential for point-of-care testing of Cu in real water samples.
构建了一种基于绿色发光铜纳米簇（Cu NCs）和红色发光银纳米簇（Ag NCs）的智能手机辅助比率荧光传感器，其中Cu NCs和Ag NCs通过静电相互作用相互结合，导致荧光共振能量从Cu NCs转移到Ag NCs。铜离子（Cu）可以淬灭Cu NCs -Ag NCs体系的红色荧光，而绿色荧光基本保持不变，从而实现对Cu的荧光比率检测。该方法对Cu具有高选择性和宽检测范围（1.0至60.0 μM）。检测限低至0.36 μM，明显低于饮用水中的最大可容忍浓度（约20 μM）。此外，智能手机可将荧光颜色转换为RGB值，表明该方法在实际水样中Cu的即时检测方面具有巨大潜力。
