总状升麻提取物可保护小鼠卵巢卵泡储备免受地塞米松的体外有害影响。
Extract of Cimicifuga racemosa (L.) Nutt protects ovarian follicle reserve of mice against in vitro deleterious effects of dexamethasone.
作者信息
Assis E I T de, Azevedo V A N, Lima Neto M F de, Costa F C, Paulino L R F M, Barroso P A A, Matos M H T, Monte A P O do, Donato M A M, Peixoto C A, Godinho A N, Freire J M O, Souza A L P, Silva J R V, Silva A W B
机构信息
Laboratório de Biotecnologia e Fisiologia da Reprodução, Universidade Federal do Ceará, Sobral, CE, Brasil.
Núcleo de Pesquisa em Experimentação Animal, Universidade Federal do Ceará, Sobral, CE, Brasil.
出版信息
Braz J Med Biol Res. 2023 Sep 22;56:e12811. doi: 10.1590/1414-431X2023e12811. eCollection 2023.
The present study aims to investigate if Cimicifuga racemosa (L.) Nutt extract (CIMI) reduces deleterious effects of dexamethasone (DEXA) in ovaries cultured in vitro. Mouse ovaries were collected and cultured in DMEM+ only or supplemented with 5 ng/mL of CIMI, or 4 ng/mL DEXA, or both CIMI and DEXA. The ovaries were cultured at 37.5°C in 5% CO2 for 6 days. Ovarian morphology, follicular ultrastructure, and the levels of mRNA for Bax, Bcl-2, and Caspase-3 were evaluated. The results showed that DEXA reduced the percentage of morphologically normal follicles, while CIMI prevented the deleterious effects caused by DEXA. In addition, DEXA negatively affected the stromal cellular density, while CIMI prevented these adverse effects. Ovaries cultured with DEXA and CIMI showed similar levels of mRNA for Bax, Bcl-2, and Caspase-3 compared to those cultured in control medium, while ovaries cultured with DEXA had increased expression of the above genes. Additionally, the ultrastructure of the ovaries cultured with CIMI was well preserved. Thus, the extract of CIMI was able to prevent the deleterious effects caused by DEXA on cultured mouse ovaries.
本研究旨在探讨升麻提取物（CIMI）是否能减轻地塞米松（DEXA）对体外培养卵巢的有害影响。收集小鼠卵巢，分别在仅含DMEM+的培养基中培养，或添加5 ng/mL CIMI的培养基中培养，或添加4 ng/mL DEXA的培养基中培养，或在同时添加CIMI和DEXA的培养基中培养。卵巢在37.5°C、5%二氧化碳条件下培养6天。评估卵巢形态、卵泡超微结构以及Bax、Bcl-2和Caspase-3的mRNA水平。结果显示，DEXA降低了形态正常卵泡的百分比，而CIMI可预防DEXA所致的有害影响。此外，DEXA对基质细胞密度有负面影响，而CIMI可预防这些不良反应。与在对照培养基中培养的卵巢相比，在同时添加DEXA和CIMI的培养基中培养的卵巢，其Bax、Bcl-2和Caspase-3的mRNA水平相似，而在仅添加DEXA的培养基中培养的卵巢，上述基因的表达增加。此外，用CIMI培养的卵巢超微结构保存良好。因此，CIMI提取物能够预防DEXA对培养的小鼠卵巢造成的有害影响。
Zotero 插件