Cronly-Dillon J R, Stafford C A
Brain Res. 1986 Nov;395(1):13-23. doi: 10.1016/s0006-8993(86)80003-8.
Variations in retinal and tectal growth activity, during regrowth of the goldfish retinotectal projection, were monitored by measuring the rates of incorporation of [14C]leucine into soluble protein and tubulin-enriched fractions at different times after crushing the optic nerves. Other experiments tested for growth-modulating interactions between tectum and retina. Here we studied how the absence of one of these structures (i.e. tectal ablation or eye removal) affected the profile of biosynthetic activity in the other. Experiments were also conducted on groups of fish in which the tectum was reinnervated by a half-retina (either half-nasal (1/2 N) or half-temporal (1/2 T) retina). This was done to ascertain if growth interactions between retina and tectum display any position-dependent differences that may be relevant to retinotopic ordering during regeneration. Our studies have revealed that: the retina and tectum of 1/2 T and 1/2 N groups differ in their growth responses during regeneration of the visual pathway: the tectum may exert a stimulatory and at other times an inhibitory influence on retinal protein synthesis; and retina and tectum display a bimodal profile of biosynthetic activity during regeneration that coincides with two stages of increased cell division (primarily glia) which other workers have found occurs in the tectum and tract during regeneration of the retinotectal projection. Indeed it seems there may be a link between this glial proliferation and the neurotrophic and guiding influences which tectum and retina exert upon one another during regeneration.
在金鱼视网膜 - 顶盖投射再生过程中，通过测量视神经切断后不同时间[14C]亮氨酸掺入可溶性蛋白和富含微管蛋白的组分的速率，监测视网膜和顶盖生长活性的变化。其他实验测试了顶盖和视网膜之间的生长调节相互作用。在此，我们研究了缺失其中一个结构（即顶盖切除或眼球摘除）如何影响另一个结构中的生物合成活性概况。还对顶盖由半视网膜（半鼻侧（1/2 N）或半颞侧（1/2 T）视网膜）重新支配的鱼群进行了实验。这样做是为了确定视网膜和顶盖之间的生长相互作用是否显示出任何与再生过程中的视网膜拓扑排序相关的位置依赖性差异。我们的研究表明：在视觉通路再生过程中，1/2 T组和1/2 N组的视网膜和顶盖在生长反应上存在差异；顶盖可能对视网膜蛋白合成有时起刺激作用，有时起抑制作用；并且视网膜和顶盖在再生过程中呈现出双峰生物合成活性概况，这与其他研究人员发现的视网膜 - 顶盖投射再生过程中顶盖和神经束中细胞分裂增加（主要是神经胶质细胞）的两个阶段相吻合。实际上，这种神经胶质细胞增殖与顶盖和视网膜在再生过程中相互施加的神经营养和引导影响之间似乎可能存在联系。
