一种使用葡萄糖脱氢酶的葡萄糖快速动力学检测方法。
A rapid kinetic assay for glucose using glucose dehydrogenase.
作者信息
Price C P, Spencer K
出版信息
Ann Clin Biochem. 1979 Mar;16(2):100-5. doi: 10.1177/000456327901600120.
PMID:37797
Abstract
An automated kinetic assay for the determination of glucose in blood is described. The method employs the enzyme glucose dehydrogenase in the presence of mutarotase, with nicotinamide adenine dinucleotide as hydrogen acceptor. The analytical parameters of the method are determined and the flexibility of the method in relation to sample volume and sensitivity is discussed. Finally, the method is compared with automated glucose oxidase and hexokinase procedures.
摘要
本文描述了一种用于测定血液中葡萄糖的自动动力学分析方法。该方法在变旋酶存在的情况下使用葡萄糖脱氢酶，以烟酰胺腺嘌呤二核苷酸作为氢受体。确定了该方法的分析参数，并讨论了该方法在样品体积和灵敏度方面的灵活性。最后，将该方法与自动葡萄糖氧化酶法和己糖激酶法进行了比较。
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A rapid kinetic assay for glucose using glucose dehydrogenase.一种使用葡萄糖脱氢酶的葡萄糖快速动力学检测方法。
Ann Clin Biochem. 1979 Mar;16(2):100-5. doi: 10.1177/000456327901600120.
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